Electrophysiological effects of dynorphin peptides on hippocampal pyramidal cells in rat.
Electrophysiological effects of dynorphin peptides on hippocampal pyramidal cells in rat.
复制标题
强啡肽对大鼠海马锥体细胞的电生理作用。
DOI：
10.1016/0014-2999(85)90286-9
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发表时间：
1985
影响因子：
5
通讯作者：
Walker,JM
中科院分区：
文献类型：
--
作者：
Moises,HC;Walker,JM
本实验采用大鼠海马锥体细胞外单细胞记录技术，观察了强啡肽和几种结构相关肽对海马锥体细胞活动的影响。强啡肽，应用磁共振或气动压力，产生了剂量依赖性的抑郁症的自发放电和谷氨酸诱发的放电在大多数（63%）的CA 1和CA 3测试细胞。此外，在两个细胞领域中的少数细胞对肽的反应是长时间的放电升高。强啡肽的抑制作用不被纳洛酮阻断。此外，去酪氨酸-强啡肽的管理抑制发射的锥体细胞的方式类似于母体化合物。乙基酮环唑辛产生兴奋和抑制作用的混合模式，而纳洛酮敏感性放电升高最常在应用强啡肽-（1-8）时观察到。应用[Leu 5]脑啡肽只产生锥体细胞放电的便利化。生物学意义上的非阿片类药物的行动，除了强大的阿片类药物介导的影响，可能会发生后释放的强啡肽原在海马体中的可能性。
Single-unit extracellular recording was carried out in rats to characterize the effects of dynorphin and several structurally related peptides on hippocampal pyramidal cell activity. Dynorphin, applied electrophoretically or by pneumatic pressure, produced a dose-dependent depression of both spontaneous and glutamate-evoked discharge in a majority (63%) of CA1and CA3cells tested. In addition, a small number of cells in both cellular fields responded to the peptide with a prolonged elevation in firing. The inhibitory effects of dynorphin in were not blocked by naloxone. Moreover, administration of des-tyrosine-dynorphin depressed the firing of pyramidal cells in a manner similar to that of the parent compound. Ethylketocyclazocine produced a mixed pattern of excitatory and inhibitory effects, whereas naloxone-sensitive elevations in firing were most often observed with the application of dynorphin-(1–8). Application of [Leu5]enkephalin produced only facilitations in pyramidal cell firing. The possibility is raised that biologically significant non-opiate actions, in addition to potent opiate-mediated effects, may occur upon release of pro-dynorphin peptides in the hippocampus.