Folate receptor-α is a cofactor for cellular entry by Marburg and Ebola viruses
Folate receptor-α is a cofactor for cellular entry by Marburg and Ebola viruses
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DOI：
10.1016/s0092-8674(01)00418-4
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发表时间：
2001-07-13
期刊：
影响因子：
64.5
通讯作者：
Goldsmith, MA
中科院分区：
文献类型：
--
作者：
Chan, SY;Empig, CJ;Goldsmith, MA
人类感染马尔堡（MBG）和埃博拉（EBO）病毒会导致致命的出血热。为了鉴定MBG病毒采用的细胞进入因子，用MBG糖蛋白（GP）包装的可选择假型病毒攻击用表达文库转导的非感染细胞。从表现出病毒进入重建的细胞中回收编码叶酸受体-α（FR-α）的cDNA。采用EBO假型以类似的策略回收FR-α cDNA。Jurkat细胞中的FR-α表达促进MBG或EBO进入，FR阻断剂抑制MBG或EBO的感染。最后，FR-α结合表达MBG或EBO GP的细胞并介导由MBG GP触发的合胞体形成。因此，FR-α是MBG和EBO病毒进入细胞的重要辅因子。
Human infections by Marburg (MBG) and Ebola (EBO) viruses result in lethal hemorrhagic fever. To identify cellular entry factors employed by MBG virus, noninfectible cells transduced with an expression library were challenged with a selectable pseudotype virus packaged by MBG glycoproteins (GP). A cDNA encoding the folate receptor-alpha (FR-alpha) was recovered from cells exhibiting reconstitution of viral entry. A FR-alpha cDNA was recovered in a similar strategy employing EBO pseudotypes. FR-alpha expression in Jurkat cells facilitated MBG or EBO entry, and FR-blocking reagents inhibited infection by MBG or EBO. Finally, FR-alpha bound cells expressing MBG or EBO GP and mediated syncytia formation triggered by MBG GP. Thus, FR-alpha is a significant cofactor for cellular entry for MBG and EBO viruses.