Calbindin-D28K in the nucleus of nerve growth factor-treated PC12 cells.
Calbindin-D28K in the nucleus of nerve growth factor-treated PC12 cells.
复制标题
经神经生长因子处理的 PC12 细胞核中的 Calbindin-D28K。
DOI：
10.1097/00001756-199611040-00003
复制
发表时间：
1996
期刊：
影响因子：
1.7
通讯作者：
German,DC
中科院分区：
文献类型：
--
作者：
McMahon,A;Lephart,ED;Chi,S;Lee,M;Iacopino,AM;German,DC
CARBINDIN-D 28 K（CB）是一种在神经元亚群中发现的钙结合蛋白，被认为可以缓冲细胞内钙并保护细胞免受钙诱导的神经退行性变。大鼠嗜铬细胞瘤PC 12细胞系是研究神经元发育和神经生长因子（NGF）分子作用的重要工具。使用Western和RNAse保护分析和免疫细胞化学和细胞分级技术，我们现在报告，NGF诱导CB的表达不仅在细胞质和神经突，而且在PC 12细胞的细胞核内。这些数据表明，PC 12细胞可以作为一个有用的模型系统，在其中研究CB的功能和核CB的作用。
CALBINDIN-D 28K (CB) is a calcium-binding protein found within a subset of neurons, which has been proposed to buffer intracellular calcium and to protect cells from calcium-induced neurodegeneration. The rat pheochromocytoma PC12 cell line has been an invaluable tool for studying neuronal development and characterizing the molecular actions of nerve growth factor (NGF). Using Western and RNAse protection analyses and immunocytochemical and cell fractionation techniques, we now report that NGF induces the expression of CB not only in the cytoplasm and neurites, but also within the nuclei of PC12 cells. These data indicate that PC12 cells can serve as a useful model system in which to study the functions of CB and the role of nuclear CB.