Synergistic Effect of Proanthocyanidin on the Bactericidal Action of the Photolysis of H2O2
Synergistic Effect of Proanthocyanidin on the Bactericidal Action of the Photolysis of H2O2
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DOI：
10.4265/bio.18.137
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发表时间：
2013-09-01
影响因子：
1.2
通讯作者：
Niwano, Yoshimi
中科院分区：
文献类型：
--
作者：
Ikai, Hiroyo;Nakamura, Keisuke;Niwano, Yoshimi
检测了 H2O2 光解与原花青素（一组多酚化合物）结合产生的羟基自由基生成系统的体外抗菌活性。 405 nm 光照射 H2O2 对龋齿主要病原体变形链球菌的杀菌活性在原花青素存在下增强，而原花青素本身的杀菌效果很差，且呈浓度依赖性。这种组合也被证明可以有效对抗牙周炎的主要病原体牙龈卟啉单胞菌。据推测，细菌细胞周围的原花青素经光照射产生的H2O2被光解为羟基自由基，进而影响细菌细胞的膜结构和功能，导致细菌细胞对利用H2O2光解的消毒系统的敏感性增强。目前的研究表明，H2O2 和原花青素的组合在光照射下可协同杀死细菌。
The in vitro antibacterial activity of the hydroxyl radical generation system by the photolysis of H2O2 in combination with proanthocyanidin, which refers to a group of polyphenolic compounds, was examined. Bactericidal activity of photo-irradiated H2O2 at 405 nm against Streptococcus mutans, a major pathogen of dental caries, was augmented in the presence of proanthocyanidin, whose bactericidal effect by itself was very poor, in a concentration-dependent manner. This combination was also proven effective against Porphyromonas gingivalis, a major pathogen of periodontitis. It is speculated that H2O2, generated from photo-irradiated proanthocyanidin around the bacterial cells, is photolyzed to the hydroxyl radical, which would in turn affect the membrane structure and function of the bacterial cells, resulting in augmented sensitivity of bacterial cells to the disinfection system utilizing the photolysis of H2O2. The present study suggests that the combination of H2O2 and proanthocyanidin works synergistically to kill bacteria when photo-irradiated.