Distribution of growth factors in subfoveal neovascular membranes in age-related macular degeneration and presumed ocular histoplasmosis syndrome.
Distribution of growth factors in subfoveal neovascular membranes in age-related macular degeneration and presumed ocular histoplasmosis syndrome.
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年龄相关性黄斑变性和推测的眼组织胞浆菌病综合征中黄斑中心凹下新生血管膜中生长因子的分布。
DOI：
10.1016/s0002-9394(14)72158-0
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发表时间：
1995
影响因子：
4.2
通讯作者：
Henry J. Kaplan
中科院分区：
文献类型：
--
作者：
Venkat M. Reddy;Rene L. Zamora;Henry J. Kaplan
PurposeWe进行了组织病理学和免疫组织学研究，以确定的大分子和细胞成分的黄斑下surgery.MethodsSubfoveal新生血管膜去除的时间从10例（7与年龄相关性黄斑变性和3个假定的眼组织胞浆菌病综合征）。通过光学和电子显微镜检查获得的组织以鉴定结构组分。免疫组织化学染色，然后进行与各种生长因子，包括转化生长因子-β 1，碱性成纤维细胞生长因子，血小板衍生生长因子，和表皮生长因子，以及抗体对前胶原1和磷酸酪氨酸residues. ResultsSubvavealneovascularmembranes大多数细胞视网膜色素上皮细胞和细胞类似成纤维细胞，与一些血管内皮细胞，淋巴细胞和巨噬细胞。在细胞外基质和内皮细胞中发现碱性成纤维细胞生长因子。在内皮细胞、成纤维细胞和视网膜色素上皮细胞中发现了转化生长因子β 1。前胶原1被发现在蛋白质合成成纤维细胞，和磷酸酪氨酸残基内检测成纤维细胞，内皮细胞，和视网膜色素上皮cells.ConclusionsSubfoveal新生血管膜新生血管复合物组成的视网膜色素上皮细胞，成纤维细胞，血管内皮细胞，和慢性炎症细胞。此外，转化生长因子-β 1和碱性成纤维细胞生长因子存在于主要细胞类型中，这表明在新生血管复合体的发展中可能具有致病作用。
PurposeWe performed a histopathologic and immunohistologic study to determine the macro-molecular and cellular components of subfoveal neovascular membranes removed at the time of submacular surgery.MethodsSubfoveal neovascular membranes were surgically removed from ten patients (seven with age-related macular degeneration and three with presumed ocular histoplasmosis syndrome). Tissues obtained were examined by light and electron microscopy to identify structural components. Immunohistochemical staining was then performed with monoclonal antibodies to various growth factors, including transforming growth factor-beta 1, basic fibroblast growth factor, platelet-derived growth factor, and epidermal growth factor, as well as antibodies against procollagen 1 and phosphotyrosine residues.ResultsMost cells in subfoveal neovascular membranes are retinal pigment epithelial cells and cells resembling fibroblasts, with some vascular endothelial cells, lymphocytes, and macrophages. Basic fibroblast growth factor was found in the extracellular matrix and in endothelial cells. Transforming growth factor-beta 1 was found in endothelial cells, fibroblasts, and retinal pigment epithelial cells. Procollagen 1 was found in protein-synthesizing fibroblasts, and phosphotyrosine residues were detected within fibroblasts, endothelial cells, and retinal pigment epithelial cells.ConclusionsSubfoveal neovascular membranes are neovascular complexes composed of retinal pigment epithelial cells, fibroblasts, vascular endothelial cells, and chronic inflammatory cells. Furthermore, transforming growth factor-beta 1 and basic fibroblast growth factor are present within the major cell types, which suggests a possible pathogenic role in the development of the neovascular complex.
DOI：
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发表时间：
1991
期刊：
The American journal of pathology
影响因子：
--
作者：
Rae Merwin;W. Newman;L. Dawson;BeaIl;A. Tucker;J. Madri
通讯作者：
Rae Merwin;W. Newman;L. Dawson;BeaIl;A. Tucker;J. Madri
影响因子：
4.4
作者：
R. Amin;J. Puklin;R. Frank
通讯作者：
R. Amin;J. Puklin;R. Frank
I 型原胶原羧基末端结构域的单克隆抗体使合成胶原的成纤维细胞可视化。
DOI：
10.1172/jci112707
发表时间：
1986
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
McDonald,JA;Broekelmann,TJ;Matheke,ML;Crouch,E;Koo,M;Kuhn3rd,C
通讯作者：
Kuhn3rd,C