Fluorescent probe for early mitochondrial voltage dynamics
Fluorescent probe for early mitochondrial voltage dynamics
复制标题
DOI：
10.1039/d1cc01944a
复制
发表时间：
2021-05-04
影响因子：
4.9
通讯作者：
Jimenez-Sanchez, Arturo
中科院分区：
文献类型：
--
作者：
Hernandez-Juarez, Cinthia;Flores-Cruz, Ricardo;Jimenez-Sanchez, Arturo
线粒体电压动力学在细胞健康和疾病中起着至关重要的作用。在这里，一个新的荧光探针监测线粒体早期电压的变化。缓慢渗透的探针在测量期间保留在线粒体中，以通过并入可水解的酯功能来避免来自天然膜电位的干扰。电压，局部极性，pH参数和跨膜动力学被发现是深刻相关的线粒体传感打开的方法。
Mitochondrial voltage dynamics plays a crucial role in cell healthy and disease. Here, a new fluorescent probe to monitor mitochondrial early voltage variations is described. The slowly permeant probe is retained in mitochondria during measurements to avoid interferences from natural membrane potential by incorporating an hydrolizable ester function. Voltage, local polarity, pH parameters and transmembrane dynamics were found to be deeply correlated opening a approach in mitochondrial sensing.