Survey of harbours in Newfoundland for indigenous and non-indigenous ascidians and an analysis of their cytochrome c oxidase I gene sequences
Survey of harbours in Newfoundland for indigenous and non-indigenous ascidians and an analysis of their cytochrome c oxidase I gene sequences
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DOI：
10.3391/ai.2010.5.1.5
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发表时间：
2010-03-01
影响因子：
1.6
通讯作者：
Rise, Matthew L.
中科院分区：
文献类型：
--
作者：
Callahan, Ashley G.;Deibel, Don;Rise, Matthew L.
自20世纪90年代中期以来，入侵的非本地海鞘一直是新斯科舍省和爱德华王子岛水产养殖业的重要生物污染问题。加拿大大西洋沿岸的问题物种包括柄海鞘、玻璃海鞘、施氏葡萄球菌和紫色葡萄球菌。我们在过去三年中进行的纽芬兰港口调查显示存在B。schlosseri和B.紫罗兰对两种非土著海鞘（Botryllus schlosseri和Botrylloides violaceus）和两种土著海鞘（Boltenia echinata和Halocynthia pyriformis）的COI基因部分序列进行了克隆和测序。然后，我们确定种内和种间COI序列变异。结果表明，Schlosseri葡萄孢菌和Halocynthia pyriformis与GenBank中的其他物种的序列完全一致。然而，来自Botrylloides violaceus的BLAST结果与B更相似。schlosseri than B.紫罗兰虽然没有其他的条目Boltenia echinata，它是类似的其他物种的Pyuridae家庭。在纽芬兰种群的COI序列的种内相似性为B>99.7%。violaceus，H.梨形菌和B. B.刺孢属（echinata），B为86.5-96.6%。施洛塞里。所有四个物种之间的种间相似性是
Invasive, non-indigenous ascidians have been a significant biofouling problem for the aquaculture industry in Nova Scotia and Prince Edward Island since the mid-1990's. The problematic species in Atlantic Canada include Styela clava, Ciona intestinalis, Botryllus schlosseri and Botrylloides violaceus. Newfoundland harbour surveys that we have performed over the past three years revealed the presence of B. schlosseri and B. violaceus. As of yet, neither of these species has reached invasive abundance in Newfoundland.Portions of the COI genes of two non-indigenous ascidians (Botryllus schlosseri and Botrylloides violaceus) and two indigenous ascidians (Boltenia echinata and Halocynthia pyriformis) were cloned and sequenced. We then determined intraspecific and interspecific COI sequence variation. The BLASTN results showed that Botryllus schlosseri and Halocynthia pyriformis match the other listings for these species in GenBank. However, the BLAST results from Botrylloides violaceus were more similar to B. schlosseri than B. violaceus. While there were no other entries for Boltenia echinata, it was similar to other species in the Pyuridae family.Intraspecific similarity in the COI sequence for Newfoundland populations was >99.7% for B. violaceus, H. pyriformis and B. echinata, and 86.5-96.6% for B. schlosseri. Interspecific similarities among all four species were