Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart
Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart
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DOI：
10.1038/nature07060
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发表时间：
2008-07-03
期刊：
影响因子：
64.8
通讯作者：
Pu, William T.
中科院分区：
文献类型：
--
作者：
Zhou, Bin;Ma, Qing;Pu, William T.
心脏由表达转录因子Nkx2 - 5和Isl1的心肌祖细胞形成（参考文献1和2）。这些多能祖细胞产生心肌细胞、平滑肌细胞和内皮细胞，它们是成熟心脏的主要细胞谱系（3,4）。在此我们鉴定出一种新型的心肌前体细胞，其特征是表达转录因子Wt1，且位于心外膜内——覆盖心脏的一层上皮。在正常小鼠心脏发育过程中，这些Wt1(+)前体细胞的一部分分化为功能完全的心肌细胞。Wt1(+)心外膜前体细胞来自表达Nkx2 - 5和Isl1的祖细胞，这表明它们与多能的Nkx2 - 5(+)和Isl1(+)祖细胞有共同的发育起源。这些结果确定Wt1(+)心外膜细胞是此前未被识别的心肌祖细胞，为未来利用这些祖细胞的心肌生成潜能进行心脏再生和修复奠定了基础。
The heart is formed from cardiogenic progenitors expressing the transcription factors Nkx2-5 and Isl1 ( refs 1 and 2). These multipotent progenitors give rise to cardiomyocyte, smooth muscle and endothelial cells, the major lineages of the mature heart(3,4). Here we identify a novel cardiogenic precursor marked by expression of the transcription factor Wt1 and located within the epicardium - an epithelial sheet overlying the heart. During normal murine heart development, a subset of these Wt1(+) precursors differentiated into fully functional cardiomyocytes. Wt1(+) proepicardial cells arose from progenitors that express Nkx2-5 and Isl1, suggesting that they share a developmental origin with multipotent Nkx2-5(+) and Isl1(+) progenitors. These results identify Wt1(+) epicardial cells as previously unrecognized cardiomyocyte progenitors, and lay the foundation for future efforts to harness the cardiogenic potential of these progenitors for cardiac regeneration and repair.