Capillary-based multi nanoelectrospray emitters: Improvements in ion transmission efficiency and implementation with capillary reversed-phase LC-ESI-MS
Capillary-based multi nanoelectrospray emitters: Improvements in ion transmission efficiency and implementation with capillary reversed-phase LC-ESI-MS
复制标题
DOI：
10.1021/ac701647s
复制
发表时间：
2008-01-01
影响因子：
7.4
通讯作者：
Smith, Richard D.
中科院分区：
文献类型：
--
作者：
Kelly, Ryan T.;Page, Jason S.;Smith, Richard D.
我们描述了耦合液相色谱（LC）分离与质谱（MS）使用纳米电喷雾电离（nano-ESI）多发射器。19个发射器的阵列降低了输送到每个发射器的流速，从而允许将纳米ESI的特征灵敏度增强扩展到更高流速的分离。当使用多发射器时，由于通过新设计的加热多毛细管MS入口增加的电离效率和改善的离子转移效率，来自掺入人血浆样品的蛋白质的胰蛋白酶片段的信号平均增加11倍。此外，当使用多发射器配置时，LC峰信噪比增加了约7倍。发射器阵列的低死体积保留了峰形和分辨率，用于使用2 μ L/min的总流速的稳健的毛细管LC分离。
We describe the coupling of liquid chromatography (LC) separations with mass spectrometry (MS) using nanoelectrospray ionization (nano-ESI) multiemitters. The array of 19 emitters reduced the flow rate delivered to each emitter, allowing the enhanced sensitivity that is characteristic of nano-ESI to be extended to higher flow rate separations. The signal for tryptic fragments from proteins spiked into a human plasma sample increased 11-fold on average when the multiemitters were employed, due to increased ionization efficiency and improved ion transfer efficiency through a newly designed heated multicapillary MS inlet Additionally, the LC peak signal-to-noise ratio increased similar to 7-fold when the multiemitter configuration was used. The low dead volume of the emitter arrays preserved peak shape and resolution for robust capillary LC separations using total flow rates of 2 mu L/min.