MOLECULAR ANALYSIS OF THE WAXY LOCUS OF ZEA-MAYS
MOLECULAR ANALYSIS OF THE WAXY LOCUS OF ZEA-MAYS
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DOI：
10.1007/bf00333960
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发表时间：
1986-01-01
期刊：
影响因子：
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通讯作者：
SAEDLER, H
中科院分区：
文献类型：
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作者：
KLOSGEN, RB;GIERL, A;SAEDLER, H
野生型蜡质(WX+)基因座的结构是通过对基因组克隆和几乎全长的cDNA克隆的序列分析确定的。编码区全长3,718个碱基，由14个外显子和13个小内含子组成。外显子和启动子区域富含G/C(60%-80%)。到目前为止，分析的所有三个蜡转录本都显示了不同的聚腺苷酸化位点和相应的聚腺苷酸化信号。其中最小的一个有2,263个核苷酸大小。Northern印迹分析表明，该基因的组织特异性表达是转录调控的结果。所分析的所有转座元件诱导的蜡质突变的插入位点都被精确地定位在该基因座内。对成熟的wx+蛋白进行N-末端测序，鉴定出一个由72个氨基酸残基组成的玉米淀粉体特异性转运肽。
The structure of the wild-typewaxy (wx+) locus was determined by sequence analysis of both a genomic and an almost full-size cDNA clone. The coding region comprises 3,718 bp and is composed of 14 exons and 13 small introns. The exons and the promoter region are G/C rich (60%–80%). All threewaxytranscripts analysed so far reveal different polyadenylation sites and corresponding polyadenylation signals. The smallest of these mRNAs has a size of 2,263 nucleotides. Northern blot analysis suggests that the tissue-specific expression of the locus is due to transcriptional control. The insertion sites of all transposable element inducedwaxymutations analysed have been mapped precisely within the locus. N-terminal sequencing of the maturewx+protein leads to the identification of a maize amyloplast-specific transit peptide of 72 aminoacid residues.