The ability of the chick wing bud to regulate positional disparity along the anterior-posterior axis.
The ability of the chick wing bud to regulate positional disparity along the anterior-posterior axis.
复制标题
鸡翅芽调节沿前后轴位置差异的能力。
DOI：
10.1016/0012-1606(87)90242-9
复制
发表时间：
1987
影响因子：
2.7
通讯作者：
Fallon,JF
中科院分区：
文献类型：
--
作者：
Dvorak,L;Fallon,JF
当将楔形翅芽组织添加到宿主翅芽中时，移植物和宿主之间存在位置差异，从而导致骨骼重复（L. E. Iten 和 D. J. Murphy 1980）Dev Biol.75,373–385。重复的极性可以通过极坐标模型预测，从而解释位置差异导致了重复。为了确定位置差异是否单独导致重复，而不增加组织的并发症，我们将外胚层和中胚层的楔形物围绕翼芽内的近远轴旋转。沿远端边缘测量 200-800 μm 的楔形物在第 20-22 阶段旋转 180°，相对于芽反转前后轴和背腹轴。这导致了位置差异，类似于 Iten 和 Murphy (1980) 在不添加组织的情况下实现的效果。我们发现，不涉及偏振区组织的旋转会产生正常的翅膀或缺少某些远端部分的翅膀，完全位于偏振区内的组织的旋转也是如此。然而，当极化区中胚层移位，使得极化和非极化组织并置时，大多数手术都会产生极化骨骼复制。我们的数据表明，位置差异本身不会导致鸡翅芽中的骨骼重复，除非极化区组织发生移位。此外，这些数据表明，在偏振区不移动的情况下，小鸡翼芽可以在面对较大的位置差异时调节形成正常的翅膀骨架。最后，我们的结果可以通过所提出的极化形态发生素对移位细胞导致复极化的作用来解释。
When wedges of wing bud tissue are added to a host wing bud so there is positional disparity between graft and host, skeletal duplications result (L. E. Iten and D. J. Murphy 1980)Dev Biol.75,373–385. The polarity of the duplications is predictable by the polar coordinate model, leading to the interpretation that the positional disparity caused the duplications. To determine whether positional disparity alone causes duplications, without the complication of added tissue, we rotated wedges of ectoderm and mesoderm around the proximodistal axiswithinthe wing bud. Wedges measuring 200–800 μm along the distal edge were rotated 180° at stages 20–22, reversing the anteroposterior and dorsoventral axes relative to the bud. This caused positional disparity, similar to that achieved by Iten and Murphy (1980), without the addition of tissue. We found that rotations involving no polarizing zone tissue produced normal wings or wings lacking some distal parts, as did rotations of tissue lying entirely within the polarizing zone. However, when polarizing zone mesoderm was displaced, so that polarizing and nonpolarizing tissues were juxtaposed, a majority of the operations produced polarized skeletal duplications. Our data demonstrate that positional disparity alone does not cause skeletal duplications in the chick wing bud, unless polarizing zone tissue is displaced. Further, these data demonstrate that the chick wing bud can regulate to form a normal wing skeleton in the face of large positional disparity, provided that the polarizing zone is not moved. Finally, our results may be explained by the action of the proposed polarizing morphogen on the displaced cells causing repolarization.