Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae.
Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae.
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U14 小核 RNA (snR128) 的消耗会破坏酿酒酵母中 18S rRNA 的产生。
DOI：
10.1128/mcb.10.3.1145-1152.1990
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发表时间：
1990
影响因子：
5.3
通讯作者：
Fournier,MJ
中科院分区：
文献类型：
--
作者：
Li,HD;Zagorski,J;Fournier,MJ
研究表明，抑制酿酒酵母必不可少的核核小核RNA （snRNA）基因可导致18S rRNA的产生受损。在128个核苷酸snR128的snRNA物种（snR128）中观察到，这种效应在snR128基因抑制开始后的一代内就很明显，并且与snRNA的消耗密切相关。在分析过程中，18S RNA与25S RNA的稳态质量比下降了8倍。脉冲追踪实验结果显示，这种不平衡的基础是18S RNA及其20S前体的积累不足。这种效应似乎是由于在定义20S物种的位点上35S rRNA转录物的加工受损，加上不稳定中间物的快速周转。讨论了所观察到的效应的可能依据。对于结构相关的酵母snRNA和来自两栖动物和哺乳动物的4.5S杂rna，提出了一个通用的U14命名。
Repression of an essential nucleolar small nuclear RNA (snRNA) gene ofSaccharomyces cerevisiaewas shown to result in impaired production of 18S rRNA. The effect, observed for an snRNA species of 128 nucleotides (snR128), was evident within one generation after the onset ofSNR128gene repression and correlated well with depletion of the snRNA. The steady-state mass ratio of 18S RNA to 25S RNA decreased eightfold over the course of the analysis. Results from pulse-chase assays revealed the basis of the imbalance to be underaccumulation of 18S RNA and its 20S precursor. This effect appears to result from impairment of processing of the 35S rRNA transcript at sites that define the 20S species coupled with rapid turnover of unstable intermediates. Possible bases for the effects observed are discussed. A common U14 designation is proposed for the structurally related yeast snRNA and 4.5S hybRNAs from amphibians and mammals.