Recombinant Fv-Hsp70 protein mediates neuroprotection after focal cerebral ischemia in rats.
Recombinant Fv-Hsp70 protein mediates neuroprotection after focal cerebral ischemia in rats.
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DOI：
10.1161/strokeaha.109.572537
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发表时间：
2010-03
期刊：
影响因子：
8.3
通讯作者：
Sharp FR
中科院分区：
文献类型：
--
作者：
Zhan X;Ander BP;Liao IH;Hansen JE;Kim C;Clements D;Weisbart RH;Nishimura RN;Sharp FR
本研究探讨重组Fv-Hsp70蛋白对实验性缺血性脑卒中后梗死体积和行为的影响。采用腔内缝合技术闭塞大脑中动脉（MCA），造成局灶性脑缺血。大鼠局灶性缺血2小时后存活24小时，缺血后2¼h和3 h，尾静脉注射Fv-Hsp70重组蛋白（0.5 mg / kg）或生理盐水。缺血后24 h观察感觉运动功能和梗死体积。与盐水对照组相比，局灶性脑缺血后给予Fv-Hsp70显著减少梗死体积68%，显著改善感觉运动功能。免疫印迹显示，缺血脑组织中有Fv-Hsp70，对照组中无；Fv-Hsp70抑制内源性Hsp70。Fv-Hsp70对缺血性脑卒中模型有保护作用。
This study investigated the effects of intravenous recombinant Fv-Hsp70 protein on infarction volume and behavior following experimental ischemic stroke. Focal cerebral ischemia was produced by occluding the middle cerebral artery (MCA) using the intraluminal suture technique. Rats subjected to 2 hours of focal ischemia were allowed to survive 24 h. At 2 ¼ h and 3 h after onset of ischemia, Fv-Hsp70 recombinant protein (0.5 mg / kg) or saline was injected via the tail vein. Sensory-motor function and infarction volume were assessed at 24 h following ischemia. Administration of Fv-Hsp70 following focal cerebral ischemia significantly decreased infarct volume by 68% and significantly improved sensory-motor function compared to the saline-treated control group. Western blots showed Fv-Hsp70 in ischemic but not in control brain; and Fv-Hsp70 suppressed endogenous Hsp70. Fv-Hsp70 protects ischemic brain in this experimental stroke model.