IMMUNOCYTOCHEMICAL LOCALIZATION OF DARPP-32, A DOPAMINE AND CYCLIC-AMP-REGULATED PHOSPHOPROTEIN, IN THE PRIMATE BRAIN
IMMUNOCYTOCHEMICAL LOCALIZATION OF DARPP-32, A DOPAMINE AND CYCLIC-AMP-REGULATED PHOSPHOPROTEIN, IN THE PRIMATE BRAIN
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DOI：
10.1002/cne.903230206
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发表时间：
1992-09-08
影响因子：
2.5
通讯作者：
GREENGARD, P
中科院分区：
文献类型：
--
作者：
OUIMET, CC;LAMANTIA, AS;GREENGARD, P
用免疫细胞化学方法研究了多巴胺和cAMP调节的磷酸蛋白DARPP-32在猴脑中的定位。这项研究表明，DARPP-32在黑质和腹侧被盖区接受密集多巴胺输入的区域的神经元中丰富。因此，前嗅区、伏隔核、尾状核和壳核的大部分体细胞都有DARPP-32免疫反应阳性。在尾状核，免疫反应阳性的髓接受来自未标记轴突终末的不对称接触。免疫反应体直径为10-15微米。在已知接受这些核团投射的区域，免疫反应仅限于代表轴突和轴突终末的小斑点。斑点内有免疫反应的区域包括腹侧苍白球、苍白球和黑质网状部。多巴胺能神经元本身不是免疫反应性的。大脑皮层的部分区域，特别是在颞叶皮质(VI层)，可以观察到含有中等到弱DARPP-32免疫反应性的神经元。DARPP-32阳性神经元也存在于小脑、内侧缰核、终纹和杏仁复合体床核的部分区域。DARPP-32在皮质下白质的星形胶质细胞、弓状核和正中隆起的伸展细胞中也有表达，DARPP-32可能是多巴胺能神经元的有效标记物，其中多巴胺对D-1多巴胺受体的作用是通过cAMP及其相关蛋白激酶介导的。
The localization of DARPP-32, a dopamine and cAMP-regulated phosphoprotein, has been studied in monkey brain by immunocytochemistry. This study indicates that DARPP-32 is enriched in neurons in regions receiving a dense dopamine input from the substantia nigra and ventral tegmental area. Thus, the majority of somata in the anterior olfactory area, nucleus accumbens, caudate nucleus, and putamen are immunoreactive for DARPP-32. In the caudate nucleus, immunoreactive spines receive asymmetric contacts from unlabeled axon terminals. Immunoreactive somata have diameters of 10-15-mu-m. In regions known to receive projections from these nuclei, immunoreactivity is confined to small puncta that represent axons and axon terminals. Regions in which immunoreactivity is present in puncta include the ventral pallidum, globus pallidus, and substantia nigra pars reticulata. Dopaminergic neurons themselves are not immunoreactive.Neurons containing moderate to weak immunoreactivity for DARPP-32 are observed in portions of the cerebral cortex, particularly in the temporal cortex (layer VI). DARPP-32-positive neurons are also present in the cerebellum, in the medial habenula, and in portions of the bed nucleus of the stria terminalis and amygdaloid complex. DARPP-32 immunoreactivity is also present in astrocytes in the subcortical white matter and in tanycytes in the arcuate nucleus and median eminence.DARPP-32 may be an effective marker for dopaminoceptive neurons in which the actions of dopamine on the D-1 dopamine receptor are mediated through cAMP and its associated protein kinase.