Tenrec phylogeny and the noninvasive extraction of nuclear DNA
Tenrec phylogeny and the noninvasive extraction of nuclear DNA
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DOI：
10.1080/10635150500433649
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发表时间：
2006-04-01
影响因子：
6.5
通讯作者：
Hofreiter, M
中科院分区：
文献类型：
--
作者：
Asher, RJ;Hofreiter, M
部分由于材料的稀缺，还没有发表的研究已经解决了生活和化石Tenrecidae（哺乳动物，非洲兽）的系统学分支。使用一种非侵入性的技术从博物馆标本的细胞核DNA采样，我们调查的Tenrecidae的进化，并评估Tenrecids适合模式的关系，提出其他陆地哺乳动物在马达加斯加的程度。应用几个树重建技术的核生长激素受体基因序列和形态数据的所有公认的tenrecid属支持单系马达加斯加tenrecids排除两个生活的非洲属。然而，简约和贝叶斯方法有利于化石非洲马岛和马达加斯加Geogale之间的密切关系，支持岛屿paraphyly的假设，但不是polyphyly。更一般地说，非侵入性提取技术可以以最小的风险应用于稀有/独特的标本，并通过更好地利用博物馆收藏的遗传工作，可以大大减少实地费用和自然种群的干扰。
Due in part to scarcity of material, no published study has yet cladistically addressed the systematics of living and fossil Tenrecidae ( Mammalia, Afrotheria). Using a noninvasive technique for sampling nuclear DNA from museum specimens, we investigate the evolution of the Tenrecidae and assess the extent to which tenrecids fit patterns of relationships proposed for other terrestrial mammals on Madagascar. Application of several tree-reconstruction techniques on sequences of the nuclear growth hormone receptor gene and morphological data for all recognized tenrecid genera supports monophyly of Malagasy tenrecids to the exclusion of the two living African genera. However, both parsimony and Bayesian methods favor a close relationship between fossil African tenrecs and the Malagasy Geogale, supporting the hypothesis of island paraphyly, but not polyphyly. More generally, the noninvasive extraction technique can be applied with minimal risk to rare/unique specimens and, by better utilizing museum collections for genetic work, can greatly mitigate field expenses and disturbance of natural populations.