Characterization of recombinant rabies virus carrying double glycoprotein genes
Characterization of recombinant rabies virus carrying double glycoprotein genes
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DOI：
10.1111/j.1348-0421.2006.tb03785.x
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发表时间：
2006-01-01
影响因子：
2.6
通讯作者：
Minamoto, N
中科院分区：
文献类型：
--
作者：
Hosokawa-Muto, J;Ito, N;Minamoto, N
利用克隆的RC-HL株cDNA，通过反向遗传学方法获得了携带双糖蛋白（G）基因的重组狂犬病病毒R（NPMGGL）株，并与重组RC-HL（rRC-HL）株的生物学特性进行了比较。R（NPMGGL）株在培养细胞中的病毒生长程度和对成年小鼠的毒力与rRC HL株几乎相同，而纯化的R（NPMGGL）病毒粒子的G蛋白含量和感染细胞的G蛋白表达水平比rRC HL株高1.5倍。作为R（NPMGGL）株在培养细胞中连续传代的结果，在用传代的R（NPMGGL）株感染的培养细胞中G蛋白的表达水平得以维持，并且病毒滴度随着对培养细胞的适应而升高。紫外线灭活病毒免疫小鼠的中和效价分析表明，R（NPMGGL）株的免疫原性高于rRC HL株。结果表明，携带双G基因的R（NPMGGL）株有可能成为研制狂犬病灭活疫苗的候选株。
A recombinant rabies virus carrying double glycoprotein (G) genes, R(NPMGGL) strain, was generated by a reverse genetics system utilizing cloned cDNA of the RC-HL strain, and the biological properties of the virus were compared to those of the recombinant RC-HL (rRC-HL) strain. The extents of virus growth in cultured cells and virulence for adult mice of the R(NPMGGL) strain were almost same as those of the rRC-HL strain, while G protein content of the purified R(NPMGGL) virion and G protein expression level in R(NPMGGL)-infected cells were 1.5-fold higher than those of the rRC-HL strain. As a result of serial passages of the R(NPMGGL) strain in cultured cells, the expression level of G protein in cultured cells infected with the passaged R(NPMGGL) strain was maintained and virus titers rose with adaptation to the cultured cells. Furthermore, analysis of neutralization titers in mice immunized with UV-inactivated virus suggested that the R(NPMGGL) strain had higher immunogenicity than that of the rRC-HL strain. The results suggest that the R(NPMGGL) strain carrying double G genes might be a useful candidate for development of a new inactivated rabies vaccine.