LS-X-SS RECOMBINANT INBRED STRAINS OF MICE - INITIAL CHARACTERIZATION
LS-X-SS RECOMBINANT INBRED STRAINS OF MICE - INITIAL CHARACTERIZATION
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DOI：
10.1111/j.1530-0277.1989.tb00310.x
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发表时间：
1989-04-01
期刊：
影响因子：
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通讯作者：
PETERSEN, DR
中科院分区：
文献类型：
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作者：
DEFRIES, JC;WILSON, JR;PETERSEN, DR
为了评估乙醇诱导的麻醉差异的遗传相关性，一组27个重组近交系（RI）来自“长睡眠”（LS）和“短睡眠”（SS）选择系小鼠的初始杂交。在F24和F25代中，在腹膜内注射4.1 g/kg体重剂量的乙醇后，对来自LSXSS RI品系的534和580只小鼠的样本进行了跌倒时间、睡眠时间和觉醒时血液乙醇的测试。大约2周后，还测试来自F24的小鼠的体温降低和血液乙醇消除率（β 60）。RI菌株的平均乙醇诱导的睡眠时间分数之间的差异很大（范围从36到171分钟），占所观察到的方差的50%以上。由于世代，性别，菌株内的窝，菌株和世代之间的相互作用的影响也是显着的，但占总方差相对较小的比例。这些数据的定量遗传学分析表明，睡眠时间评分和多基因的差异，然而，在白化病（c）基因座的等位基因差异可能有一个多效性的影响。睡眠时间与觉醒时的血液乙醇（-0.79）之间以及注射后60分钟的体温与β 60（+0.48）之间的遗传相关性是显著的。由于LSXSS RI菌株之间的差异很大，高度可靠，它们应该是有价值的动物模型，用于测试更多的搜索关于乙醇诱导麻醉的个体差异的病因学的假设。
In order to assess the genetic correlates of differences in ethanol-induced anesthesia, a set of 27 recombinant inbred (RI) strains was derived from an initial cross of the "long-sleep" (LS) and "short-sleep" (SS) selected lines of mice. In generations F24 and F25, samples of 534 and 580 mice from the LSXSS RI strains were tested for fall time, sleep time, and blood ethanol at awakening subsequent to intraperitoneal injection of a 4.1-g/kg body weight dose of ethanol. Approximately 2 weeks later, mice from F24 were also tested for body temperature lowering and blood-ethanol elimination rate (.beta.60). Differences among the average ethanol-induced sleep-time scores of the RI strains are large (ranging from 36 to 171 min) and account for over 50% of the observed variance. Effects due to generation, sex, litters within strains, and the interaction between strain and generation are also significant, but account for relatively small proportions of the total variance. Quantitative genetic analyses of these data suggest that differences in sleep-time scores and polygenic; however, allelic differences at the albino (c) locus may have a pleiotropic effect. Genetic correlations between sleep time and blood ethanol at awakening (-0.79) and between body temperature 60 min after injection and .beta.60 (+0.48) are significant. Because differences among the LSXSS RI strains are large and highly reliable, they should be valuable animal models for testing more searching hypotheses about the etiology of individual differences in ethanol-induced anesthesia.