5-lipoxygenase pathway promotes cell proliferation in human glioma cell lines.
5-lipoxygenase pathway promotes cell proliferation in human glioma cell lines.
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DOI：
10.5414/npp28445
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发表时间：
2009-11
影响因子：
1.1
通讯作者：
K. Ishii;M. Zaitsu;N. Yonemitsu;Y. Kan;Y. Hamasaki;M. Matsuo
中科院分区：
文献类型：
--
作者：
K. Ishii;M. Zaitsu;N. Yonemitsu;Y. Kan;Y. Hamasaki;M. Matsuo
OBJECTIVE 5-lipoxygenase (5-LO) is a key enzyme in the synthesis of leukotrienes (LTs), that might promote carcinogenesis. We investigated 5-LO expression and examined whether the 5-LO pathway is associated with the proliferation of human brain tumors. METHODS We immunohistochemically evaluated the profile of 5-LO expression in various types of brain tumors obtained from 42 patients, and examined the proliferative effects of the 5-LO pathway in human glioma cell lines using a proliferation assay. RESULTS Immunohistochemistry of glioblastomas, astrocytomas, meningiomas, medulloblastomas, craniopharyngiomas, ependymomas, neurinomas, oligodendrogliomas, malignant lymphomas, dysembryoplastic neuroepithelial and metastatic brain tumors revealed 5-LO expression in the cytoplasm and nuclei or nuclear envelopes of tumor cells. The 5-LO inhibitor A861 and the LTA4 hydrolase inhibitor Bestatin dose-dependently suppressed the proliferation of A172 cells, a glioma cell line. CONCLUSIONS We confirmed the expression of 5-LO in various human brain tumors and demonstrated the partial suppression of tumor growth by inhibitors of the 5-LO-LTA4 hydrolase pathway in human glioma cell lines. The 5-LO-LTA4 pathway might play roles in the proliferation of human glioma cells.
