In vivo imaging of cerebral glucose metabolism informs on subacute to chronic post-stroke tissue status - A pilot study combining PET and deuterium metabolic imaging.
In vivo imaging of cerebral glucose metabolism informs on subacute to chronic post-stroke tissue status - A pilot study combining PET and deuterium metabolic imaging.
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DOI：
10.1177/0271678x221148970
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发表时间：
2023-05
影响因子：
6.3
通讯作者：
Dijkhuizen, Rick M.
中科院分区：
文献类型：
--
作者：
Meerwaldt, Anu E.;Straathof, Milou;Oosterveld, Wija;van Heijningen, Caroline L.;van Leent, Mandy M. T.;Toner, Yohana C.;Munitz, Jazz;Teunissen, Abraham J. P.;Daemen, Charlotte C.;van der Toorn, Annette;van Vliet, Gerard;van Tilborg, Geralda A. F.;De Feyter, Henk M.;de Graaf, Robin A.;Hol, Elly M.;Mulder, Willem J. M.;Dijkhuizen, Rick M.
关键词：
急性缺血性卒中后再通治疗可恢复脑灌注。然而，一个显着的子集的患者有不良的结果，这可能是由于大脑能量代谢的破坏。为了评估再通后亚急性和慢性葡萄糖代谢的变化，我们在C57 BL/6小鼠短暂大脑中动脉闭塞（tMCAO）60分钟后应用了两种互补的成像技术，氟脱氧葡萄糖（FDG）正电子发射断层扫描（PET）和氘（2 H）代谢成像（SPECT）。用FDG PET测量的葡萄糖摄取在tMCAO后48小时降低，并在11天后恢复到基线值。结果显示，tMCAO后48小时，病变区域葡萄糖供应有效，乳酸产生增加，谷氨酸/谷氨酰胺合成减少，其程度取决于卒中严重程度。11天后，氧化代谢进一步降低。免疫组化显示病变内和周围有显著的胶质细胞活化，这可能在观察到的代谢谱中起作用。我们的研究结果表明，成像（改变）活跃的葡萄糖代谢的再灌注脑卒中病变和周围可以提供大量的信息（继发性）缺血后脑组织的病理生理变化。
Recanalization therapy after acute ischemic stroke enables restoration of cerebral perfusion. However, a significant subset of patients has poor outcome, which may be caused by disruption of cerebral energy metabolism. To assess changes in glucose metabolism subacutely and chronically after recanalization, we applied two complementary imaging techniques, fluorodeoxyglucose (FDG) positron emission tomography (PET) and deuterium (2H) metabolic imaging (DMI), after 60-minute transient middle cerebral artery occlusion (tMCAO) in C57BL/6 mice. Glucose uptake, measured with FDG PET, was reduced at 48 hours after tMCAO and returned to baseline value after 11 days. DMI revealed effective glucose supply as well as elevated lactate production and reduced glutamate/glutamine synthesis in the lesion area at 48 hours post-tMCAO, of which the extent was dependent on stroke severity. A further decrease in oxidative metabolism was evident after 11 days. Immunohistochemistry revealed significant glial activation in and around the lesion, which may play a role in the observed metabolic profiles. Our findings indicate that imaging (altered) active glucose metabolism in and around reperfused stroke lesions can provide substantial information on (secondary) pathophysiological changes in post-ischemic brain tissue.