Human nevocellular nevus cells are surrounded by basement membrane components. Immunohistologic studies of human nevus cells and melanocytes in vivo and in vitro.
Human nevocellular nevus cells are surrounded by basement membrane components. Immunohistologic studies of human nevus cells and melanocytes in vivo and in vitro.
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人类痣细胞痣细胞被基底膜成分包围。
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
Barbara A. Gilchrest
中科院分区：
文献类型：
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作者：
M. Yaar;Woodley Dt;Barbara A. Gilchrest
皮肤中的真皮痣细胞被类似于基底膜（BM）的电子致密沉积物包围，并且表皮黑素细胞位于表皮BM上。使用针对各种BM成分的抗体，我们已经确定，在体内痣细胞周围的BM样结构含有IV型胶原蛋白，层粘连蛋白和BM-1蛋白聚糖，类似于全身的BM，但不是大疱性类天疱疮抗原或大疱性表皮获得性抗原，这些抗原是表皮BM中存在的角质形成细胞相关蛋白。此外，在体外，痣细胞和黑素细胞来源于成人供体显示细胞内和细胞外纤连蛋白，BM-1蛋白聚糖，IV型胶原蛋白，和层粘连蛋白。相反，在相同的培养条件下维持的新生黑素细胞不显示这些BM组分，强调供体年龄对细胞行为的影响。这些数据表明，真皮痣细胞在体内制造BM，就像某些其他神经嵴来源的细胞一样。培养的痣细胞和黑素细胞合成BM成分的明显共享能力，加上其他先前指出的体外行为和形态学相似性，表明这些细胞类型是非常密切相关的;并且存在于体内的形态学或组织化学差异是环境影响的结果，而不是内在差异。
Dermal nevus cells in the skin are surrounded by electron-dense deposits resembling a basement membrane (BM), and epidermal melanocytes rest on the epidermal BM. Using antibodies directed against various BM components, we have determined that the BM-like structure surrounding nevus cells in vivo contains type IV collagen, laminin, and BM-1 proteoglycan, analogous to BM throughout the body, but not bullous pemphigoid antigen or epidermolysis bullosa acquisita antigen that are keratinocyte-associated proteins present in the epidermal BM. Moreover, in vitro, both nevus cells and melanocytes derived from adult donors display intracellular and extracellular fibronectin, BM-1 proteoglycan, type IV collagen, and laminin. In contrast, newborn melanocytes maintained under identical culture conditions display none of these BM components, emphasizing the influence of donor age on cell behavior. The data suggest that dermal nevus cells manufacture a BM in vivo, as do certain other neural crest-derived cells. The apparent shared ability of cultured nevus cells and melanocytes to synthesize BM components, coupled with other previously noted behavioral and morphologic similarities in vitro, suggests that these cell types are very closely related; and that morphologic or histochemical differences present in vivo are the result of environmental influences rather than intrinsic differences.