Quantitating glomerular endothelial fenestration: an unbiased stereological approach.
Quantitating glomerular endothelial fenestration: an unbiased stereological approach.
复制标题
定量肾小球内皮开窗：一种无偏见的体视学方法。
DOI：
10.1159/000327075
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发表时间：
2011
影响因子：
4.2
通讯作者：
Mauer,Michael
中科院分区：
文献类型：
--
作者：
Najafian,Behzad;Mauer,Michael
Background/AimsGlomerular endothelial cells are fenestrated, allowing for especially high transcellular hydraulic conductivity. Current knowledge about endothelial fenestration structural changes in disease conditions is limited, partly due to the absence of robust methodologies to quantitate these structures. Herein, we propose a novel method for estimating the percentage of endothelial fenestration.MethodsAn unbiased stereological method based on contiguity of two phases and surface area density estimation using isotropic uniform random line probes was developed. A line grid for intercept counting and classifying endothelial coverage of fenestrated versus non-fenestrated areas was designed. The method was applied to renal biopsies from 15 patients with Fabry disease and 9 normal living kidney donor controls.ResultsThe percentage of glomerular capillary endothelial coverage which was fenestrated was lower in Fabry patients (43±12%) versus controls (53±9%; p= 0.047). The fraction of endothelial surface which was fenestrated was greater on the peripheral versus mesangial zones of the capillary walls in both Fabry patients (p= 0.00002) and controls (p= 0.0005).ConclusionThe proposed method provides an unbiased tool to quantitate endothelial fenestration changes in glomeruli. The practical example introduced showed reduced glomerular endothelial fenestration in Fabry nephropathy.
