Fluorescence-based resource for semiautomated genomic analyses using microsatellite markers.
Fluorescence-based resource for semiautomated genomic analyses using microsatellite markers.
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使用微卫星标记进行半自动基因组分析的基于荧光的资源。
DOI：
10.1006/geno.1994.1628
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发表时间：
1994
期刊：
影响因子：
4.4
通讯作者：
Hudson,JR
中科院分区：
文献类型：
--
作者：
Levitt,RC;Kiser,MB;Dragwa,C;Jedlicka,AE;Xu,J;Meyers,DA;Hudson,JR
为了促进高效的半自动化方法在基因组分析中的实际应用，我们开发了一种基于荧光的微卫星标记资源。90个高度多态性的微卫星标记相结合，提供了一个快速，准确，高效的初始全基因组筛选系统。这些标记平均每33 cM间隔，平均杂合性为81%（范围65-94%），覆盖22个常染色体和X和Y染色体。不到10%的基因组位于最近标记的20 cM之外。由于该基因组分析系统与使用同时四色荧光检测系统的自动片段分析仪完全兼容，因此可以同时检测5组18个标记。这种多重检测提供了每台仪器每天1944个基因型的通量。该系统将在许多临床和研究应用中非常有益，包括连锁，癌症遗传学，法医学和细胞遗传学。
To facilitate the practical application of highly efficient semiautomated methods for general application in genomic analyses, we have developed a fluorescence-based microsatellite marker resource. Ninety highly polymorphic microsatellite markers were combined to provide a rapid, accurate, and highly efficient initial genome-wide screening system. These markers are spaced on average every 33 cM, with a mean heterozygosity of 81% (range 65-94%), covering 22 autosomes and the X and Y chromosomes. Less than 10% of the genome lies beyond 20 cM of the nearest marker. Since this genomic analysis system is fully compatible with automated fragment analyzers using simultaneous four-color fluorescence-based detection systems, the 5 groups of 18 markers can be detected concurrently. This multiplex detection provides a throughput of 1944 genotypes daily per instrument. This system will be highly beneficial in a number of clinical and research applications including linkage, cancer genetics, forensics, and cytogenetics.