Alterations in ribonucleic acid synthesis by chromium (III).
Alterations in ribonucleic acid synthesis by chromium (III).
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铬 (III) 改变核糖核酸合成。
DOI：
10.1016/s0162-0134(00)80157-9
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发表时间：
1981
影响因子：
3.9
通讯作者：
M. Taniyama
中科院分区：
文献类型：
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作者：
S. Okada;H. Ohba;M. Taniyama
与其他无机金属相比，研究了 Cr(III) 对由小鼠肝脏分离的 DNA 和染色质指导的体外 RNA 合成的影响。在添加大肠杆菌 RNA 聚合酶之前，与 DNA 或染色质一起孵育时，1 mM 的 CrCl3 显着刺激 RNA 合成，而其他金属则抑制 RNA 合成。即使在 1 μM CrCl3 下，Cr(III) 也会对 DNA 或染色质指导的 RNA 合成产生这种刺激。 DNA 和染色质的 Cr(III) 复合物也显示出模板活性显着增强。另一方面，当 RNA 聚合酶与 Cr(III) 预孵育时，在 DNA 和染色质指导下的 RNA 合成中观察到显着的抑制。在具有内源性 RNA 聚合酶的离体小鼠肝细胞核中，Cr(III) 刺激 Mg2+ 激活的 RNA 合成，但不刺激 Mn2+-(NH4)2SO4 激活的合成。这些结果表明 Cr(III) 可能改变或调节哺乳动物的基因表达。
The effect of Cr(III) on in vitro RNA synthesis directed by DNA and chromatin isolated from mouse liver was investigated in comparison with other inorganic metals. At 1 mM, CrCl3significantly stimulated RNA synthesis when incubated with DNA or chromatin prior to the addition ofEscherichia coliRNA polymerase, while other metals inhibited it. This stimulation by Cr(III) was caused even at 1 μM CrCl3on either DNA- or chromatin-directed RNA synthesis. The Cr(III)-complexes of DNA and chromatin also showed significantly enhanced template activities. On the other hand, when RNA polymerase was preincubated with Cr(III), a remarkable inhibition was observed in RNA synthesis directed by both DNA and chromatin. In isolated mouse liver nuclei with endogenous RNA polymerases, Cr(III) stimulated Mg2+-activated RNA synthesis but not Mn2+-(NH4)2SO4-activated synthesis. These results suggest that Cr(III) may alter or regulate gene expressions in mammals.