Structure and properties of the precipitates formed from condensed solutions of the revised simulated body fluid
Structure and properties of the precipitates formed from condensed solutions of the revised simulated body fluid
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DOI：
10.1002/jbm.a.10127
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发表时间：
2003-11-01
影响因子：
4.9
通讯作者：
Dorozhkin, SV
中科院分区：
文献类型：
--
作者：
Dorozhkina, EI;Dorozhkin, SV
使用等于人血浆离子浓度的2、4、8和12倍的Kokubo修订的模拟体液（rSBF）的水溶液进行沉淀实验。代替Hepes，通过CO2鼓泡或加入HCl将溶液pH调节至所需值7.40 +/-0.02。实验在密闭的塑料容器中进行，在37.0 +/-0.2 ℃下持续振荡72小时。然后，过滤悬浮液，收集沉淀物并分析。结果表明，随着rSBF浓度的增加，沉淀物的结构和化学成分都发生了很大的变化。磷酸盐取代碳酸盐（虽然钙和镁的量保持不变）和结晶度降低是最重要的修改中发现的沉淀物形成的rSBF的高度浓缩的解决方案。(C)2003 Wiley Periodicals，Inc.
Precipitation experiments with aqueous solutions of the Kokubo's revised simulated body fluid (rSBF) equal to 2, 4, 8, and 12 times the ionic concentration of human blood plasma were performed. Instead of Hepes, solution pH was adjusted to the desired value of 7.40 +/- 0.02 by either bubbling of CO2 or addition of HCl. The experiments were performed in tightly closed plastic vessels kept at 37.0 +/- 0.2degreesC for 72 h under permanent shaking. Afterward, the suspensions were filtrated, and the precipitates were collected and analyzed. The results revealed that increasing the concentration of rSBF resulted in great changes in both the structure and the chemical composition of the precipitates. Phosphate substitution for carbonate (although the amounts of calcium and magnesium remained unchanged) and crystallinity decreasing were the most important modifications found in the precipitates formed from the highly condensed solutions of rSBF. (C) 2003 Wiley Periodicals, Inc.