Antimicrobial efficacy of a human β-defensin-3 peptide using an Enterococcus faecalis dentine infection model.
Antimicrobial efficacy of a human β-defensin-3 peptide using an Enterococcus faecalis dentine infection model.
复制标题
使用粪肠球菌牙本质感染模型研究人 β-防御素-3 肽的抗菌功效。
DOI：
--
复制
发表时间：
2013
影响因子：
5
通讯作者：
L. Spångberg
中科院分区：
文献类型：
--
作者：
Jong;Young;K. Kum;S. Han;Seok;Blythe Kaufman;Jin Jiang;Qiang Zhu;K. Safavi;L. Spångberg
目的
评估人β-防御素-3（HBD 3）肽对粪肠球菌生物膜的抗菌效力。
方法
用E.粪链球菌ATCC 29212培养3周。氢氧化钙水溶液糊剂（n = 12，CH）、2%氯己定凝胶（n = 12，CHX）、HBD 3肽凝胶（n = 12）和生理盐水（n = 12）作为实验组进行测试。对照组为不匹配的肽凝胶组（n = 12，MP）和灭菌但未接种的块组（n = 12）。给药1周后，分别于根管内200 μ m和400 μm处取牙本质标本。孵育72小时后，通过光密度（OD）的分光光度分析评估细菌生长。采用重复测量方差分析和Tukey事后检验进行统计分析。
结果
在两个深度，HBD 3组与CH或CHX组相比具有显著更低的OD值（P < 0.05）。CH组与MP组和生理盐水组在两个深度上均无显著差异（P > 0.05）。各组牙本质内层（200 μm）和外层（400 μm）的OD值差异均无统计学意义（P > 0.05）。
结论
HBD 3肽段对E.感染牙本质块中的粪菌生物膜。
AIM
To assess the antibacterial efficacy of a human β-defensin-3 (HBD3) peptide against Enterococcus faecalis biofilms.
METHODOLOGY
Standardized human dentine blocks were infected with E. faecalis ATCC 29212 for 3 weeks. Aqueous calcium hydroxide paste (n = 12, CH), a 2% chlorhexidine gel (n = 12, CHX), an HBD3 peptide gel (n = 12) and saline (n = 12) were tested as experimental groups. A mismatched peptide gel group (n = 12, MP) and sterilized but noninoculated block group (n = 12) were included as controls. After 1 week of medication, the dentinal samples at the depth of 200 and 400 μm were collected from medicated canal lumens. Bacterial growth was assessed by spectrophotometric analysis of optical density (OD) after 72 h of incubation. Statistical analysis was performed with repeated-measures anova and Tukey's post hoc test.
RESULTS
The HBD3 group was associated with significantly lower OD values (P < 0.05) than the CH or CHX groups at both depths. The CH group did not differ significantly from MP or Saline group at either depth (P > 0.05). There was no significant difference (P > 0.05) in the OD values of the inner (200 μm) and outer (400 μm) dentinal samples for any group.
CONCLUSIONS
The HBD3 peptide inhibited the growth of E. faecalis biofilms in infected dentine blocks.