Enhanced thermal stability of laccase immobilized on monolayer-modified nanoporous Au
Enhanced thermal stability of laccase immobilized on monolayer-modified nanoporous Au
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DOI：
10.1016/j.matlet.2011.08.017
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发表时间：
2012-01-01
影响因子：
3
通讯作者：
Mabuchi, Mamoru
中科院分区：
文献类型：
--
作者：
Hakamada, Masataka;Takahashi, Masaki;Mabuchi, Mamoru
采用硝酸脱合金法制备了纳米多孔Au，并将其与4-氨基苯硫酚自组装膜修饰，以提高固定化漆酶的高温活性。固定化漆酶在>45 ℃时比游离漆酶表现出更高的活性。SAM表面修饰大大提高了固定化漆酶的热稳定性和重复使用性。例如，在50 ℃孵育2小时后，当固定在SAM修饰的纳米多孔Au上时，观察到漆酶活性几乎没有降解。这表明纳米孔结构和SAM协同阻止了漆酶的构象变化，从而提高了漆酶的高温活性。(C)2011 Elsevier B. V.保留所有权利。
Nanoporous Au was fabricated by the dealloying of Au-Ag alloy in nitric acid, and was modified with a self-assembled monolayer (SAM) of 4-aminothiophenol, for the enhancement of high-temperature activity of immobilized laccase. Immobilized laccase exhibited much higher activity than that of free laccase at >45 degrees C. SAM surface modification greatly improved the thermal stability and reusability of immobilized laccase. For example, little degradation in laccase activity when immobilized on SAM-modified nanoporous Au was observed after 2 h incubation at 50 degrees C. This suggested the nanoporous structure and SAM synergistically prevented the conformational change of laccase, and resulted in the enhancement of high-temperature activity. (C) 2011 Elsevier B.V. All rights reserved.