Hybridization Detection of Osmium Tetroxide Bipyridine‐Labeled DNA and RNA on Heated Gold Wire Electrodes
Hybridization Detection of Osmium Tetroxide Bipyridine‐Labeled DNA and RNA on Heated Gold Wire Electrodes
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DOI：
10.1002/elan.201200460
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发表时间：
2013-02
期刊：
影响因子：
3
通讯作者：
M. Jacobsen;G. Flechsig
中科院分区：
文献类型：
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作者：
M. Jacobsen;G. Flechsig
我们报告了捕获探针修饰的加热金丝电极上不同核酸的杂交检测。我们比较了三种核酸靶标：DNA、尿嘧啶结合DNA和RNA。三种核酸靶均能用四氧化锇联吡啶标记，与固定化的DNA捕获探针杂交，然后用方波伏安法检测。在大多数情况下，加热金电极而不是整个本体杂交溶液导致改进的杂交效率。其原因可能是加热丝电极周围的热微搅拌效应。选择性也得到改善。可以区分DNA和尿嘧啶缀合的DNA靶的错配。然而，RNA链中的错配由于相对稳定的二级结构而更难以检测。
We report about hybridization detection of different nucleic acids on capture probe-modified heated gold wire electrodes. We have compared three kinds of nucleic acid targets: DNA, uracil-conjugated DNA, and RNA. All three sorts of nucleic acids targets could be labeled with osmium tetroxide bipyridine, hybridized with immobilized DNA capture probes and then detected by square-wave voltammetry. Heating the gold electrode instead of the entire bulk hybridization solution leads to improved hybridization efficiency in most cases. The reason could be found in a thermal micro-stirring effect around the heated wire electrode. Also selectivity was improved. Mismatches could be discriminated for DNA and uracil-conjugated DNA targets. Mismatches in RNA strands, however, are more difficult to detect due to relatively stable secondary structures.