A SIMPLIFIED METHOD FOR CRYOPRESERVATION OF PLASMODIUM-FALCIPARUM FROM CONTINUOUS INVITRO CULTURES
A SIMPLIFIED METHOD FOR CRYOPRESERVATION OF PLASMODIUM-FALCIPARUM FROM CONTINUOUS INVITRO CULTURES
复制标题
DOI：
10.1080/00034983.1983.11811685
复制
发表时间：
1983-01-01
影响因子：
--
通讯作者：
MILLER, H
中科院分区：
文献类型：
--
作者：
CHRISTOFINIS, GJ;MILLER, H
从体外连续培养物中获得并储存在-185 ℃下的寄生有恶性疟原虫的人红细胞。温度为70 ℃。C为几个月，都被成功回收了在生长培养基RPMI 1640中使用10%甘油或10%二甲基亚砜，发现其作为冷冻保存剂同样有效。这种简化的方法在储存前或回收寄生虫后不需要离心或特殊培养基。通过启动新的连续培养物在体外监测寄生虫的活力。
Human erthrocytes parasitized with P. .intg.alciparum, obtained from in vitro continuous cultures and stored at -185.degree. C and at 70.degree. C for several mo., were successfully recovered. Ten percent glycerol or 10% dimethyl sulphoxide in growth medium RPMI 1640 were used and found equally effective as cryopreservatives. This simplified method does not require centrifugation or special media before storage or after recovery of the parasites. The viability of the parasites is monitored in vitro by initiating new continuous cultures.