Independent metal-binding features of recombinant metallothioneins convergently draw a step gradation between Zn- and Cu-thioneins
Independent metal-binding features of recombinant metallothioneins convergently draw a step gradation between Zn- and Cu-thioneins
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DOI：
10.1039/b904953c
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发表时间：
2009-05-01
期刊：
影响因子：
3.4
通讯作者：
Atrian, Silvia
中科院分区：
文献类型：
--
作者：
Bofill, Roger;Capdevila, Merce;Atrian, Silvia
通过多年的系统研究，收集了大约20种来自进化不同生物体的重组金属硫蛋白(MTS)的金属结合行为的数据，在这里进行了全面的分析。对体内和体外四个独立的金属结合特征的考虑揭示了金属硫蛋白与金属结合特征的分级，这被认为与一个强有力的新分类显著一致：锌和铜硫蛋白之间的逐步分级。这一列表中的中间位置被一组多价MT占据，表现出一种合并的锌/铜硫蛋白特征，将适合一般金属处理目的。相反，极端的位置分别由那些可能进化为履行与锌或铜相关的特殊生理角色的MT占据。总体而言，分析的趋势允许对NIT分类标准提出化学和生物上合理的新思考。
Data on the metal-binding behaviour of circa 20 recombinant metallothioneins (MTs) from evolutionary divergent organisms, gathered after years of systematic research, are here comprehensively analyzed. The consideration of four independent in vivo and in vitro metal-binding features reveals a gradation of the metal-binding character of the MTs considered that significantly coincides in a robust new classification: a stepwise gradation between Zn- and Cu-thioneins. The intermediate positions in this list are Occupied by a group of polyvalent MTs, exhibiting a merging Zn-/Cu-thionein character that would suit general metal handling purposes. In contrast, the extreme positions are respectively occupied by those MTs that Would have evolved to fulfil specialized Zn- or Cu-related physiological roles. Overall, the analyzed trends allow the proposal of a chemically- and biologically-sound new reflection on NIT classification criteria.