Towards a nomenclatural clarification of the Peltigera ponojensis/monticola clade including metagenomic sequencing of type material and the introduction of P. globulata Miadl. & Magain sp. nov.
Towards a nomenclatural clarification of the Peltigera ponojensis/monticola clade including metagenomic sequencing of type material and the introduction of P. globulata Miadl. & Magain sp. nov.
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DOI：
10.1017/s0024282923000373
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发表时间：
2023-09-01
期刊：
影响因子：
1.4
通讯作者：
Lutzoni，Francois
中科院分区：
文献类型：
--
作者：
Miadlikowska，Jolanta;Magain，Nicolas;Lutzoni，Francois
球花黄藤& Magain，正式描述了Peltigera组P. ponojensis/monticola种复合体中的一新种。这个分支以前被临时命名为Peltigera sp. 17。在秘鲁和厄瓜多尔的高海拔地区发现了它暴露在阳光下的干燥栖息地。Peltigera globulata可以很容易地通过其不规则的球形边缘被厚的白色粉霜覆盖而被识别，有点类似于P. soredians的sorediate叶状体边缘，P. soredians是来自Peltigera组的另一个南美物种。ITS 1的高变区（ITS 1-HR）在Peltigera组的物种中通常是高度可变的，对于P. ponojensis/monticola复合体中的物种鉴定没有诊断价值。然而，没有显着水平的基因流中检测到8个谱系代表一个分支的推定物种（包括P. globulata）在这个复杂的。山地疫霉正模标本的ITS序列。（1979年收集）和P. soredians Vitik.（1981年采集）和选择型标本。W. Dodge（1941年采集）和P. aubertii C. W. Dodge（1952年收集）通过桑格和Illumina宏基因组测序成功获得。这些序列的BLAST结果表明，P. monticola的模式标本福尔斯P. monticola/ponojensis 7进化枝，代表P. monticola s。str.，并证实模式标本P. aubertii福尔斯属于以前根据形态学鉴定为P. aubertii的一个分支。来自模式标本的ITS序列，表面上类似于P. globulata，证实了它在P. rufescens分支中的位置。最后，我们发现，P. anarctica的名称被错误地应用于P. ponojensis/monticola分支中的一个谱系。模式标本的ITS序列代表了P. rufescens分支中的一个谱系，它是P. ponojensis/monticola分支的姐妹。
Peltigera globulata Miadl. & Magain, a new species in the P. ponojensis/monticola species complex of section Peltigera, is formally described. This clade was previously given the interim designation Peltigera sp. 17. It is found in sun-exposed and xeric habitats at high altitudes in Peru and Ecuador. Peltigera globulata can be easily recognized by its irregularly globulated margins covered mostly by thick, white pruina, somewhat resembling the sorediate thallus margins of P. soredians, another South American species from section Peltigera. The hypervariable region of ITS1 (ITS1-HR), which is in general highly variable among species of section Peltigera, does not have diagnostic value for species identification within the P. ponojensis/monticola complex. Nevertheless, no significant level of gene flow was detected among eight lineages representing a clade of putative species (including P. globulata) within this complex. ITS sequences from the holotype specimens of P. monticola Vitik. (collected in 1979) and P. soredians Vitik. (collected in 1981) and lectotype specimens of P. antarctica C. W. Dodge (collected in 1941) and P. aubertii C. W. Dodge (collected in 1952) were successfully obtained through Sanger and Illumina metagenomic sequencing. BLAST results of these sequences revealed that the type specimen of P. monticola falls within the P. monticola/ponojensis 7 clade, which represents P. monticola s. str., and confirmed that the type specimen of P. aubertii falls within a clade identified previously as P. aubertii based on morphology. The ITS sequence from the type specimen of P. soredians, which superficially resembles P. globulata, confirms its placement in the P. rufescens clade. Finally, we discovered that the name P. antarctica was erroneously applied to a lineage in the P. ponojensis/monticola clade. The ITS sequence from the type specimen of P. antarctica represents a lineage within the P. rufescens clade, which is sister to the P. ponojensis/monticola clade.