Protein degradation by Prevotella intermedia and Actinomyces meyeri supports the growth of non-protein-cleaving oral bacteria in serum
Protein degradation by Prevotella intermedia and Actinomyces meyeri supports the growth of non-protein-cleaving oral bacteria in serum
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DOI：
10.1111/j.1600-051x.1997.tb00768.x
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发表时间：
1997-05-01
影响因子：
6.7
通讯作者：
vanderHoeven, JS
中科院分区：
文献类型：
--
作者：
Jansen, HJ;vanderHoeven, JS
口腔细菌的蛋白水解活性被认为在牙科疾病的病因学中起重要作用。细菌衍生的蛋白酶可能导致组织破坏，并且可能通过降解免疫球蛋白和补体来损害宿主防御。退化的牙周组织和组织液很可能构成脓肿中营养物质的重要来源。来自血清的组织液富含蛋白质而缺乏碳水化合物，这表明蛋白质的分解和氨基酸的发酵是产生微生物生长所需能量的关键步骤。进行蛋白质水解裂解成多肽（蛋白质降解的第一步）的口腔细菌物种的数量与绝大多数产肽酶物种相比相对较少。因此，在这项研究中，我们研究了生长促进作用的蛋白酶生产的物种，如中间普雷沃氏菌和放线菌meyeri对一些非蛋白酶生产的细菌在混合培养物中的生长。我们使用血清作为脓肿微生物群落的所谓天然基质的替代品。采用毛细管电泳法研究了血清蛋白的降解。在不产生蛋白酶的迟缓真杆菌、具核梭杆菌、微小消化链球菌和中间链球菌的单一和混合培养物中发现生长不良。根据混合培养物的生长是肽限制性的假设，在混合培养物中中间青霉的存在强烈地增强了这4个物种的生长。中间青霉在链霉蛋白酶消化的血清中的生长增强表明该生物体在血清中的肽限制性生长，尽管其产生蛋白酶。结果表明，麦氏放线菌的单培养生长较差。与此相反，A. meyeri在混合培养中生长良好，其存在刺激了F. nucleatum和P. micros，表明协同关系。使用每个种属的一种代表性菌株研究单一和混合培养物的生长。因此，具有选择的独特菌株的风险很小。蛋白酶抑制剂减少牙龈卟啉单胞菌，变黑普雷沃氏菌，和P. intermedia在胰蛋白胨-酵母提取物培养基中的生长，和没有，IgG。我们的研究表明，蛋白酶生产微生物在口腔细菌在人血清中的混合培养物中起着关键作用，通过提供多肽的生长。这可能解释了它们与牙科疾病的关系。
The proteolytic activities of oral bacteria are thought to play an important role in the aetiology of dental abscesses. Bacteria-derived proteases may contribute to tissue destruction, and are likely to impair host defence by degrading immunoglobulins and complement. Degraded periodontal tissue and tissue fluid are likely to constitute essential sources of nutrients in the abscess. Tissue fluid, which is derived from serum, is rich in protein and poor in carbohydrate, suggesting that breakdown of protein and fermentation of amino acids is a crucial step to generate energy for growth of the microflora. The number of oral bacterial species that perform hydrolytic cleavage of protein into polypeptides, the first step in protein degradation, is relatively small compared to the large majority of peptidase-producing species. In this study, we therefore investigated the growth-promoting effect of proteinase-producing species like Prevotella intermedia and Actinomyces meyeri on the growth of some non-proteinase producing bacteria in mixed cultures. We used serum as a substitute for the supposed natural substrate of the abscess microflora. The breakdown of serum proteins was investigated using capillary electrophoresis. Poor growth was found in mono- and mixed cultures of non-proteinase producing species Eubacterium lentum, Fusobacterium nucleatum, Peptostreptococcus micros and Streptococcus intermedius. The presence of P. intermedia in mixed cultures strongly enhanced growth of these 4 species, according to the hypothesis that the growth of the mixed cultures was peptide-limited. The enhanced growth of P. intermedia in pronase-digested serum indicated peptide-limited growth of this organism in serum, despite its production of proteinase. We found that growth of monocultures of Actinomyces meyeri was poor. In contrast, A. meyeri grew well in mixed cultures and its presence stimulated growth of F. nucleatum and P. micros, suggesting a synergistic relationship. The growth of mono- and mixed cultures was investigated using one representative strain of each species. Thus, there is a small risk of having selected unique strains. Proteinase inhibitors reduced the growth of Porphyromonas gingivalis, Prevotella nigrescens, and P. intermedia in trypticase peptone-yeast extract medium with, and without, IgG. Our study indicated that proteinase-producing organisms play a key role in mixed cultures of oral bacteria in human serum by providing polypeptides for growth. This may explain their association with dental abscesses.