Bacillus thuringiensis EM-A1: A novel bacterium for high concentration of ammonium elimination with low nitrite accumulation.
Bacillus thuringiensis EM-A1: A novel bacterium for high concentration of ammonium elimination with low nitrite accumulation.
复制标题
DOI：
10.1016/j.chemosphere.2023.139465
复制
发表时间：
2023-07
期刊：
影响因子：
8.8
通讯作者：
Chenyu Ding;T. He
中科院分区：
文献类型：
--
作者：
Chenyu Ding;T. He
近年来，生物消除废水中高浓度氨氮越来越受到人们的关注。然而，关于单一细菌有效消除高浓度铵的研究报道较少。在这里，苏云金芽孢杆菌 EM-A1 在 150 rpm、pH 7.2、琥珀酸钠、碳/氮比为 15、30 °C、接种量（通过 600 nm 处的吸光度测量）为 0.2 的情况下，有效消除了 NH4+-N (>99%) 和总氮 (TN) (>77%)。菌株 EM-A1 有效消除了 100 mg/L 的无机氮，最大 NH4+-N、NO3−-N 和 NO2−-N 消除率分别为 4.88、2.57 和 3.06 mg/L/h。初始浓度为 500 mg/L 和 1000 mg/L 时，NH4+-N 的消除效率分别为 99.87% 和 97.13%。消除 1000 mg/L NH4+-N 后，仅积累 0.91 mg/L NO2−-N。浓度为 5 mg/L 的外源羟胺具有毒性，会进一步抑制异养硝化和好氧反硝化 (HN-AD)。菌株 EM-A1 的 NH4+-N 和 NO2−-N 消除能力分别被超过 4 μmol/L 的 2-辛炔 (OCT) 和超过 0.5 mmol/L 的二乙基二硫代氨基甲酸酯 (DDC) 特异性抑制。超过 25 mg/L 原花青素 (PCY) 会抑制 NO3−-N 和 NO2−-N 的生物转化。结果表明，菌株EM-A1在嗜盐条件下具有HN-AD能力，在氮污染废水处理中具有巨大的应用潜力；这项研究还为该菌株的脱氮机制提供了新的见解，有助于推进环境生物技术。
The biological elimination of high concentration of ammonium from wastewater has attracted increasing attention in recent years. However, few studies on the efficient elimination of high concentration of ammonium by a single bacterium have been reported. Here, the efficient elimination of NH4+-N (>99%) and total nitrogen (TN) (>77%) were attained by Bacillus thuringiensis EM-A1 under 150 rpm at pH 7.2 with sodium succinate and a carbon/nitrogen ratio of 15 at 30 °C with an inoculum size (as measured by absorbance at 600 nm) of 0.2. Strain EM-A1 effectively eliminated 100 mg/L of inorganic nitrogen with maximal NH4+-N, NO3−-N, and NO2−-N elimination rates of 4.88, 2.57, and 3.06 mg/L/h, respectively. The elimination efficiencies of NH4+-N were 99.87% and 97.13% at initial concentrations of 500 and 1000 mg/L, respectively. Only 0.91 mg/L of NO2−-N was accumulated with the elimination of 1000 mg/L NH4+-N. A concentration of 5 mg/L exogenous hydroxylamine was toxic and further inhibited heterotrophic nitrification and aerobic denitrification (HN-AD). The NH4+-N and NO2−-N elimination capacities of strain EM-A1 were specifically inhibited by 2-Octyne (OCT) over 4 μmol/L and diethyldithiocarbamate (DDC) over 0.5 mmol/L, respectively. Above 25 mg/L procyanidin (PCY) inhibited the bioconversion of NO3−-N and NO2−-N. The results demonstrated that strain EM-A1 had HN-AD capacity under halophilic conditions, and has great potential for use in the treatment of nitrogen pollution wastewater; this study also provides new insights into this strain's nitrogen elimination mechanism, helping advance environmental biotechnology.