SUMO1/UBC9‑decreased Nox1 activity inhibits reactive oxygen species generation and apoptosis in diabetic retinopathy.
SUMO1/UBC9‑decreased Nox1 activity inhibits reactive oxygen species generation and apoptosis in diabetic retinopathy.
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SUMO1/UBC9摄取的NOX1活性抑制了糖尿病性视网膜病中的活性氧的产生和凋亡。
DOI：
10.3892/mmr.2017.8037
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发表时间：
2018-01
影响因子：
3.4
通讯作者：
You Z
中科院分区：
文献类型：
--
作者：
Hu J;Xue P;Mao X;Xie L;Li G;You Z
Diabetic retinopathy (DR) is an increasing global health concern that causes vision loss and blindness. Reactive oxygen species (ROS) are considered to be a principal cause of DR. An important source of ROS is the oxidization of NADPH. In the present study, NADPH oxidase 1 (Nox1)-expressing human retinal epithelial cell (HREC) lines were generated and infected with small ubiquitin-like modifier 1 (SUMO1) and/or ubiquitin conjugating enzyme E2 I (UBC9) lentiviral pGMLV constructs. The viabilities, apoptotic capacities and ROS production levels of the HREC lines were quantified using Hoechst 33258, annexin V/propidium iodide and dichlorodihydrofluorescein diacetate assays, respectively. Additionally, rat DR models were established. From these models, the apoptotic capacities of retinal tissues were visualized using terminal deoxynucleotidyl transferase dUTP nick end labeling assays, and the pathologies were evaluated. The mRNA and protein expression levels of SUMO1, UBC9 and Nox1 were analyzed using reverse transcription-quantitative polymerase chain reaction and western blot analyses, respectively. Compared with controls, the relative mRNA levels of SUMO1 and UBC9 were significantly upregulated, and the Nox1 levels significantly downregulated, in cells infected with SUMO1 or UBC9 alone or in combination. The ROS production and apoptosis rates of cells and retinal tissues were decreased. In addition, pathological symptoms in DR tissues improved when they were simultaneously transfected with SUMO1 and UBC9 via intraocular injection. In conclusion, the SUMO1/UBC9 axis may regulate Nox1-mediated DR by inhibiting ROS generation and apoptosis in rat and cellular model systems.
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DOI：
10.1111/febs.12866
发表时间：
2014-08
期刊：
The FEBS journal
影响因子：
--
作者：
Chhunchha B;Fatma N;Kubo E;Singh DP
通讯作者：
Singh DP
影响因子：
4.8
作者：
Hong, YL;Rogers, R;Sarge, KD
通讯作者：
Sarge, KD
影响因子：
37.3
作者：
Banskota, Suhrid;Regmi, Sushil C.;Kim, Jung-Ae
通讯作者：
Kim, Jung-Ae
影响因子：
8
作者：
Hwang, Kwang Woo;Won, Tae Joon;Park, Yongsoo
通讯作者：
Park, Yongsoo
Ubc9 与核定位信号相互作用并介导成对同源染色体蛋白 Vsx-1 的核定位，与 SUMO-1 修饰无关
DOI：
10.1073/pnas.101129698
发表时间：
2001-05-08
影响因子：
11.1
作者：
Kurtzman, AL;Schechter, N
通讯作者：
Schechter, N