Discovery of Novel microRNAs in Aging Caenorhabditis elegans.
Discovery of Novel microRNAs in Aging Caenorhabditis elegans.
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DOI：
10.1007/978-1-4939-2963-4_18
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发表时间：
2015
影响因子：
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通讯作者：
A. de Lencastre;F. Slack
中科院分区：
文献类型：
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作者：
A. de Lencastre;F. Slack
在过去几年中，深度测序技术的快速发展，以及随之而来的测序深度和成本效率的提高，为生物学中越来越广泛的应用打开了大门——从全基因组测序到ChIP-seq分析，表观基因组学和RNA转录组学调查。在这里，我们描述了深度测序的应用，以发现新的microrna和表征其差异表达在成年秀丽隐杆线虫。
The rapid development of deep sequencing technologies over the last few years and concomitant increases in sequencing depth and cost efficiencies have opened the door to a ever-widening range of applications in biology—from whole-genome sequencing, to ChIP-seq analysis, epigenomic and RNA transcriptome surveys. Here we describe the application of deep sequencing to the discovery of novel microRNAs and characterization of their differential expression during adulthood inCaenorhabditis elegans.