Quantum Dot Fluorescence Characterizes the Nanoscale Organization of T Cell Receptors for Antigen
Quantum Dot Fluorescence Characterizes the Nanoscale Organization of T Cell Receptors for Antigen
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DOI：
10.1016/j.bpj.2011.10.028
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发表时间：
2011-12-07
影响因子：
3.4
通讯作者：
Edidin, Michael
中科院分区：
文献类型：
--
作者：
Boyle, Sarah;Kolin, David L.;Edidin, Michael
表面受体聚集的变化调节细胞对配体的反应。因此，受体聚类的全局测量可用于表征细胞状态。以抗原的T细胞受体为例，我们证明了量子点闪烁的k空间图像相关光谱在数十纳米的尺度上检测T细胞受体簇，并报告了T细胞激活后簇的变化。我们的研究结果提供了一个通用的方法，在单细胞的横向组织和受体聚集的全球分析，因此可以应用时，感兴趣的细胞类型是罕见的。
Changes in the clustering of surface receptors modulate cell responses to ligands. Hence, global measures of receptor clustering can be useful for characterizing cell states. Using T cell receptor for antigen as an example, we show that k-space image correlation spectroscopy of quantum dots blinking detects T cell receptor clusters on a scale of tens of nano-meters and reports changes in clustering after T cell activation. Our results offer a general approach to the global analysis of lateral organization and receptor clustering in single cells, and can thus be applied when the cell type of interest is rare.