A role for brassinosteroids in light-dependent development of Arabidopsis
A role for brassinosteroids in light-dependent development of Arabidopsis
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DOI：
10.1126/science.272.5260.398
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发表时间：
1996-04-19
期刊：
影响因子：
56.9
通讯作者：
Chory, J
中科院分区：
文献类型：
--
作者：
Li, JM;Nagpal, P;Chory, J
虽然类固醇激素对动物发育很重要，但植物类固醇的生理作用尚不清楚。拟南芥DET2基因编码一种与哺乳动物类固醇5 α -还原酶具有显著序列一致性的蛋白质。谷氨酸204的突变是人类类固醇还原酶活性所必需的，它会消除体内DET2的活性，并导致光调节发育的缺陷，这种缺陷可以通过应用植物类固醇油菜素内酯来改善。因此，DET2可能编码了油菜素内酯生物合成途径中的还原酶，油菜素内酯可能构成了一类独特的植物激素，在高等植物的光调控发育中起着重要作用。
Although steroid hormones are important for animal development, the physiological role of plant steroids is unknown. The Arabidopsis DET2 gene encodes a protein that shares significant sequence identity with mammalian steroid 5 alpha-reductases. A mutation of glutamate 204, which is absolutely required for the activity of human steroid reductase, abolishes the in vivo activity of DET2 and leads to defects in light-regulated development that can be ameliorated by application of a plant steroid, brassinolide. Thus, DET2 may encode a reductase in the brassinolide biosynthetic pathway, and brassinosteroids may constitute a distinct dass of phytohormones with an important role in light-regulated development of higher plants.