Dynamics of the use of magnetocardiography in the study of the cardiac conduction system of the chick embryo.
Dynamics of the use of magnetocardiography in the study of the cardiac conduction system of the chick embryo.
复制标题
心磁图在鸡胚胎心脏传导系统研究中的应用动态。
DOI：
10.1002/bdr2.1777
复制
发表时间：
2020
影响因子：
2.1
通讯作者：
Wakai,RonaldT
中科院分区：
文献类型：
--
作者：
Schellpfeffer,MichaelA;Strasburger,JanetteF;Baffa,Oswaldo;Strand,SarahA;Lutter,William;Phan,Tan;Wakai,RonaldT
利用超导量子干涉仪（SQUID）对胎儿心磁图（fMCG）进行研究已经有几十年的历史，但在胚胎/胎儿动物模型中的研究还很少。本研究使用光泵磁力计（OPM），以获得fMCG在鸡embryon.MethodsWhite来航鸡胚胎进行了检查，从孵化第10 -19天。不同的检查室进行了测试，以优化胚胎的热稳定性和磁信号采集。所有检查均在磁屏蔽下进行。OPM传感器被放置在蛋壳旁边。胚胎的位置通过透照完整的蛋或超声成像的蛋与开放的空气细胞，以优化传感器的位置进行定位。对每个胚胎的原始数据进行后处理，以获得fMCG复合波形。使用五个传感器获得了完整鸡蛋的最佳成功;一个在底部，四个在鸡蛋的下周边，间隔90°，鸡蛋垂直定向，气室向上。在气囊打开的情况下使用超声成像，只需要两个传感器，一个在底部，一个在胚胎旁边。fMCGs进行了分析的心率和节奏，每一部分的PQT间期波形，PR间期，QRS波群，RR间期，QT interval.ConclusionsThis study validates作为一种动物模型，研究在一个纵向和非侵入性的方式，胎儿心脏传导系统，通过使用OPM magnetocardiography的鸡胚。
IntroductionHuman fetal magnetocardiography (fMCG) has been done for several decades to evaluate fetal arrhythmias using a superconducting quantum interference device (SQUID) magnetometer, but there is little work in embryonic/fetal animal models. This study uses an optically‐pumped magnetometer (OPM) to obtain an fMCG in the chick embryo.MethodsWhite Leghorn chick embryos were examined from incubation Day #10–19. Different examination chambers were tested to optimize embryonic thermal stability and magnetic signal acquisition. All examinations were done with magnetic shielding. The OPM sensors were placed next to the egg shell. The embryo's position was localized by transilluminating the intact egg or ultrasound imaging the egg with an open air cell to optimize sensor placement. The raw data for each embryo was postprocessed to obtain a fMCG composite waveform.ResultsfMCG's were obtained in embryos from Day #12 to 19. The best success with intact eggs was obtained using five sensors; one at the bottom and four around the lower perimeter of the egg at 90° intervals with the egg oriented vertically and the air cell up. Using ultrasound imaging with the air cell open only two sensors were necessary, one at the bottom and one laterally next to the embryo. fMCGs were analyzed for heart rate and rhythm, each portion of the PQRST waveform, and the PR interval, QRS complex, RR interval, and QT interval.ConclusionsThis study validates the chick embryo as an animal model to study in a longitudinal and noninvasive fashion the fetal cardiac conduction system by using OPM magnetocardiography.