PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element
PU.1 expression is modulated by the balance of functional sense and antisense RNAs regulated by a shared cis-regulatory element
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DOI：
10.1101/gad.1654808
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发表时间：
2008-08-01
影响因子：
10.5
通讯作者：
Tenen, Daniel G.
中科院分区：
文献类型：
--
作者：
Ebralidze, Alexander K.;Guibal, Florence C.;Tenen, Daniel G.
转录因子PU.1是造血的重要调节因子;精确的表达水平对于正常造血发育和抑制白血病至关重要。我们在这里表明，非编码反义RNA是适当剂量PU.1的重要调节剂。反义RNA和正义RNA受共同的进化上保守的顺式调控元件调控，我们可以证明反义RNA通过调节mRNA翻译抑制PU.1表达。我们认为，这种反义RNA可能在许多基因的调控中起重要作用，并且可能是迄今为止功能未知的大量重叠互补转录本的原因。
The transcription factor PU.1 is an important regulator of hematopoiesis; precise expression levels are critical for normal hematopoietic development and suppression of leukemia. We show here that noncoding antisense RNAs are important modulators of proper dosages of PU.1. Antisense and sense RNAs are regulated by shared evolutionarily conserved cis-regulatory elements, and we can show that antisense RNAs inhibit PU.1 expression by modulating mRNA translation. We propose that such antisense RNAs will likely be important in the regulation of many genes and may be the reason for the large number of overlapping complementary transcripts with so far unknown function.