Surfactant-Free Suspension Polymerization of Hydrophilic Monomers with an Oil-in-Water System for the Preparation of Microparticles Toward the Selective Isolation of Tumor Cells
Surfactant-Free Suspension Polymerization of Hydrophilic Monomers with an Oil-in-Water System for the Preparation of Microparticles Toward the Selective Isolation of Tumor Cells
复制标题
使用水包油系统进行亲水性单体的无表面活性剂悬浮聚合，用于制备微粒以选择性分离肿瘤细胞
DOI：
10.1039/d2ma00129b
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发表时间：
2022
影响因子：
5
通讯作者：
Tanaka Masaru
中科院分区：
文献类型：
--
作者：
Nishimura Shin-nosuke;Nishida Kei;Shiomoto Shohei;Tanaka Masaru
循环肿瘤细胞（CTC）来源于原发性肿瘤或单核细胞病灶，并且存在于肿瘤患者的血流中。我们开发了血液相容性聚（2-甲氧基乙基丙烯酸酯）（PMEA）的聚合物液滴，其选择性地在肿瘤细胞中积聚。大于细胞大小的PMEA微粒具有作为CTC捕获装置的平台而不需要使用抗体的潜力。在此，这些微粒，以及几个，其他类型的微粒组成的亲水性单体，通过无表面活性剂的悬浮聚合制备的，和它们的选择性分离能力对肿瘤细胞的捕获通过比较研究进行了评估。该微粒具有光滑且极纯的表面，适合于评估与肿瘤细胞的相互作用力。粘附于PMEA微粒的人血小板的数量明显低于粘附于其它聚合物微粒的血小板的数量。有趣的是，以1%交联率制备的PMEA微粒与肿瘤细胞的相互作用比其他聚合物微粒更强。此外，与其他聚合物微粒相比，PMEA微粒能够在动态条件下从细胞悬浮液中有效回收肿瘤细胞。这些结果提供了对PMEA微粒作为不使用抗体的CTC捕获平台的可能适用性的见解。
Circulating tumor cells (CTCs) are derived from a primary tumor or monastic foci, and are found in the bloodstream of patients with tumors. We developed polymer droplets of blood-compatible poly(2-methoxyethyl acrylate) (PMEA) that selectively accumulate in tumor cells. PMEA microparticles, which are larger than the size of cells, have potential as a platform for CTC capture devices without the need to use antibodies. Herein, these microparticles, as well as several, other types of microparticles composed of hydrophilic monomers, were prepared by surfactant-free suspension polymerization, and their selective isolation abilities toward the capture of tumor cells were evaluated via comparative studies. The microparticles possessed smooth and extremely pure surfaces suitable for evaluating the interaction force with tumor cells. The number of human platelets adhered to the PMEA microparticles was clearly lower than the number of platelets adhered to the other polymer microparticles. Interestingly, the PMEA microparticles prepared with a 1% crosslinking ratio showed stronger interactions with tumor cells than the other polymer microparticles. In addition, the PMEA microparticles enabled the efficient recovery of tumor cells from cell suspensions under dynamic conditions in comparison with the other polymer microparticles. These results provide insights into the possible applicability of PMEA microparticles as a platform for CTC capture without using antibodies.