Cross-regional scale pollution of freshwater biofilms unveiled by antibiotic resistance genes
Cross-regional scale pollution of freshwater biofilms unveiled by antibiotic resistance genes
复制标题
抗生素抗性基因揭示淡水生物膜的跨区域规模污染
DOI：
10.1016/j.scitotenv.2021.151835
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发表时间：
2022-03-04
影响因子：
9.8
通讯作者：
Habimana，Olivier
中科院分区：
文献类型：
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作者：
Yao，Yuan;Liu，Zongbao;Habimana，Olivier
目前还没有关于淡水生物膜中精氨酸生成物分布的全面全球概况。我们利用宏基因组学方法揭示了来自四个国家38个采样点的96个淡水生物膜样品中ARG的多样性，丰度，可转移性和宿主。大量ARG与杆菌肽、多药、多粘菌素、大环内酯-林可酰胺-链阳性菌素（MLS）、氨基糖苷类、β-内酰胺类、氯霉素、磺胺类和四环素耐药相关，与人用或兽用常用抗生素谱一致。正如预期的那样，淡水生物膜栖息地中的抗性基因组受地理位置和人类足迹的显著影响。基于网络分析揭示的共现模式，提出mdtC、kdpE和demrB作为淡水生物膜中ARG的指示因子，可用于定量评价其他46种共现ARG亚型的丰度。宏基因组组装分析表明，所确定的ARG由超过46个细菌门，包括各种病原体，这大大扩展了淡水生物膜中抗性组多样性的知识。我们的研究指出了生物膜在窝藏ARG中的核心作用。研究结果将有助于加深对淡水生境中的精氨酸生成物分布的了解，从而加强对精氨酸生成物的全球环境风险评估和管理。
A comprehensive global profile of the distribution of ARGs in freshwater biofilms is lacking. We utilized metagenomic approaches to reveal the diversity, abundance, transferability and hosts of ARGs in 96 freshwater biofilm samples from 38 sampling sites across four countries. The abundant ARGs were associated with bacitracin, multidrug, polymyxin macrolide-lincosamide-streptogramin (MLS) aminoglycoside, β-lactam, chloramphenicol, sulfonamide and tetracycline resistance, consistent with the spectrum of antibiotics commonly used in human or veterinary medicine. As expected, the resistome in freshwater biofilm habitats was significantly influenced by geographical location and human footprint. Based on the co-occurrence pattern revealed by network analysis,mdtC,kdpE, andemrBwere proposed as ARG indicators in freshwater biofilms that can be used to evaluate the abundance of 46 other co-occurring ARG subtypes quantitatively. Metagenomic assembly analysis revealed that the identified ARGs were hosted by more than 46 bacterial phyla, including various pathogens, which greatly expands the knowledge of resistome diversity in freshwater biofilms. Our study points to the central roles of biofilms in harbouring ARGs. The results could enhance understanding the distribution of ARGs in freshwater habitats, thereby strengthening the global environmental risk assessment and management of ARGs.