Olive Oil Polyphenol Oleuropein Inhibits Smooth Muscle Cell Proliferation
Olive Oil Polyphenol Oleuropein Inhibits Smooth Muscle Cell Proliferation
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DOI：
10.1016/j.ejvs.2010.12.021
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发表时间：
2011-06-01
影响因子：
5.7
通讯作者：
Sumpio, B.
中科院分区：
文献类型：
--
作者：
Abe, R.;Beckett, J.;Sumpio, B.
目的：地中海饮食，橄榄油含量高，与冠状动脉疾病的风险降低有关。本研究的目的是确定橄榄苦苷，橄榄油中的多酚之一，对血管平滑肌细胞（SMC）增殖的影响在vitro.Design：This is an experimental study.Materials and Methods：牛血管平滑肌细胞培养在100 μ M橄榄苦苷的存在下。在第1、3和5天，计数细胞数。流式细胞仪检测细胞周期。结果：橄榄苦苷对细胞增殖有明显的抑制作用，抑制率达92%。细胞周期分析显示，与未处理组相比，橄榄苦苷处理将细胞阻滞在G1-S期。在G1期调节因子中，视网膜母细胞瘤蛋白、细胞周期蛋白D、p21和p27不受橄榄苦苷的影响，但细胞外信号调节激酶1/2（ERK 1/2）的激活受到抑制。结论：橄榄苦苷通过阻滞细胞周期G1期和S期而抑制平滑肌细胞增殖，这一作用可能受ERK 1/2的调节。这些结果表明，橄榄油消费可能通过抑制SMC增殖对心血管死亡率产生有益影响的机制。(C)2010年欧洲血管外科学会。由爱思唯尔有限公司出版。保留所有权利。
Objectives: The Mediterranean diet, with a high content of olive oil, is associated with a reduced risk of coronary artery disease. The aim of this study was to determine the effect of oleuropein, one of the polyphenols in olive oil, on vascular smooth muscle cell (SMC) proliferation in vitro.Design: This was an experimental study.Materials and methods: Bovine vascular SMCs were cultured in the presence of 100 mu M of oleuropein. On days 1, 3 and 5, cell number was counted. Cell cycle analysis was performed by flow cytometry. Cell cycle regulators were assessed by immunoblotting.Results: Cell proliferation in the presence of oleuropein was significantly inhibited by 92%. Cell cycle analysis revealed that oleuropein treatment blocked cells in the G1-S phase compared with the non-treated group. Among G1 phase regulators, retinoblastoma protein, cyclinD, p21 and p27 were not affected by oleuropein, but extracellular signal-regulated kinase 1/2 (ERK1/2) activation was inhibited. Growth of SMC treated with 100 mu of the mitogen-activated protein (MAP) kinase/ERK kinase 1 (MEK1) inhibitor P098059 was also significantly inhibited by 70%.Conclusions: Oleuropein inhibited SMC proliferation through a cell cycle block between the G1 and the S phases, which may be regulated by ERK1/2. These results suggest a mechanism by which olive oil consumption may have beneficial effects on cardiovascular mortality by inhibiting SMC proliferation. (C) 2010 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.