The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation
The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation
复制标题
碱性功能单体辅助牛血清白蛋白表面印迹超顺磁性聚合物的制备及其在蛋白质分离中的应用
DOI：
10.1016/j.chroma.2011.03.069
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发表时间：
2011-06-03
影响因子：
4.1
通讯作者：
Zhang, Yu-Kui
中科院分区：
文献类型：
--
作者：
Gai, Qing-Qing;Qu, Feng;Zhang, Yu-Kui
目前，小蛋白质印迹报道较多，因为大蛋白质分子印迹因其体积大和结构复杂而面临挑战。本工作以普通单体（N-异丙基丙烯酰胺）为基础，在碱性功能单体（N-[3-（二甲氨基）丙基]-甲基丙烯酰胺）的辅助下，采用原子转移自由基聚合（ATRP）方法成功合成了牛血清白蛋白（BSA）表面印迹磁性聚合物，为基于ATPR在温和的反应条件下印迹更大的靶蛋白提供了可实现的尝试。与非印迹聚合物相比，BSA 印迹聚合物对 BSA 表现出更高的吸附容量和选择性。竞争吸附测试表明BSA印迹聚合物对混合物中的BSA具有更好的选择性吸附和识别性能。将获得的 BSA 印迹聚合物应用于牛血清，也显示出对 BSA 的选择性。另外，采用传统的PEG/硫酸盐两相水溶液作为吸附BSA的洗脱液，并与普通的NaCl洗脱液进行比较。 (C) 2011 Elsevier B.V. 保留所有权利。
Currently, small proteins imprinting are more reported since large proteins molecular imprinting faces challenge due to their bulk size and complex structure. In this work, bovine serum albumin (BSA) surface-imprinted magnetic polymer was successfully synthesized based on atomic transfer radical polymerization (ATRP) method in the presence of common monomer (N-isopropylacrylamide) with the assistant of basic functional monomer (N-[3-(dimethylamino)propyl]-methacrylamide), which provides a achievable attempt for imprinting larger target proteins based on the ATPR with the mild reaction conditions. The BSA-imprinted polymer exhibited higher adsorption capacity and selectivity to BSA over the non-imprinted polymer. Competitive adsorption tests indicated the BSA-imprinted polymer had better selective adsorption and recognition properties to BSA in the mixture. The obtained BSA-imprinted polymer was applied to bovine serum, which also showed selectivity to BSA. In addition, a conventional aqueous two-phase solution of PEG/sulphate was used as elution for adsorbed BSA, which was compared with common NaCl elution. (C) 2011 Elsevier B.V. All rights reserved.