Promoter analysis and chromosomal mapping of human EBAG9 gene
Promoter analysis and chromosomal mapping of human EBAG9 gene
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DOI：
10.1006/bbrc.2000.2920
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发表时间：
2000-07-05
影响因子：
3.1
通讯作者：
Inoue, S
中科院分区：
文献类型：
--
作者：
Ikeda, K;Sato, M;Inoue, S
人EBAG 9先前使用CpG-基因组结合位点克隆被鉴定为雌激素应答基因（Watchman等人，（1998）Mel. Cell. 18：442-449）。最近发现EBAG 9与癌细胞RCAS 1相同。与免疫逃逸有关的表面抗原。本研究分离并分析了人EBAG 9基因的5 ′侧翼区。我们确定了转录起始位点，它与起始元件YYCAYYYY具有同源性，并发现TATA基序缺失。使用MCF-7乳腺癌细胞进行的5 '侧翼区的缺失分析表明，含有ERE的序列-86至-36具有基础水平的启动子活性，并且上游富含CC的区域正调节活性。含有ERE的EBAG 9 prometer荧光素酶报告基因可对雌激素产生反应，电泳迁移率变动分析显示ER α与ERE结合。此外，荧光原位杂交分析表明，人EBAG 9基因位于染色体8 q23，其在肿瘤中经常扩增。这些发现表明，人类EBAG 9可能作为雌激素应答基因参与致癌作用，（C）2000学术出版社。
The human EBAG9 was previously identified as an estrogen responsive gene using CpG-genomic binding site cloning (Watanate et al, (1998) Mel. Cell. Biol. 18: 442-449). Recently it was revealed that the EBAG9 is identical with RCAS1 which is a cancer cell. surface antigen implicated in immune escape. Here, we isolated and analyzed the 5'-flanking region of human EBAG9 gene. We determined transcription initiation site, which has a homology with an initiator element YYCAYYYY, and found that TATA motif was absent. Deletion analysis of the 5'-flanking region using MCF-7 breast cancer cells indicated that the sequences -86 to -36 containing the ERE had the basal level of promoter activity and the upstream CC-rich region positively regulated the activity. EBAG9 pro meter luciferase reporters containing the ERE could respond to estrogen, and electrophoretic mobility shift assay showed that ER alpha bound to the ERE. Moreover, fluorescent in situ hybridization analysis has shown that the human EBAG9 gene is located at chromosome 8q23 which is frequently amplified in tumors. These findings suggest that the human EBAG9 might be involved in carcinogenesis as an estrogen responsive gene, (C) 2000 Academic Press.