Analyzing red blood cell-deformability distributions
Analyzing red blood cell-deformability distributions
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DOI：
10.1006/bcmd.2002.0528
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发表时间：
2002-05-01
影响因子：
2.3
通讯作者：
Grimbergen, CA
中科院分区：
文献类型：
--
作者：
Dobbe, JGG;Hardeman, MR;Grimbergen, CA
背景红细胞（RBC）必须变形以通过微循环的最小毛细血管。用于测量RBC变形性的可用技术通常提供平均变形性的指示。后者可以通过轻微的整体变形能力降低或通过存在一小部分刚性细胞来降低。这两种情况之间的区别可以用RBC变形性分布（RBC-DD）来进行。方法.本文探讨了红细胞DD的健康人和细胞异常的机械性能（镰状细胞病，透析患者，椭圆形红细胞增多症，和栽培的热带疟疾）。用自动流变仪测量分布，该流变仪使用先进的图像分析技术来获得大量经受简单剪切流的单个细胞的变形指数。结果健康志愿者的RBC-DD接近正态分布。在研究的患者中，分布明显不同，平均值、标准差或两者均发生显著变化。变形能力分布（DD）可以定性和定量地评估亚变形和超变形细胞部分的存在。在椭圆形红细胞增多症中，与正常细胞相比，细胞相对于流线的取向不同。这会导致DD出现偏差。结论. RBC-DD是建立具有异常变形性的亚群的强有力的代表。低变形和高变形细胞的分数和DD的标准差是评估RBC变形性改变的新的和优秀的定量参数。(C)2002 Elsevier Science（美国）。
Background. Red blood cells (RBCs) must deform to pass the smallest capillaries of the microcirculation. Available techniques for measuring RBC deformability often provide an indication of the mean deformability. The latter may be decreased either by a slight overall deformability reduction or by the presence of a small fraction of rigid cells. A distinction between these two cases can be made with a RBC-deformability distribution (RBC-DD). Methods. This paper explores RBC-DDs of healthy individuals and of cells with anomalous mechanical properties (sickle cell disease, dialysis patients, elliptocytosis, and cultivated malaria tropica). The distributions were measured with an automated rheoscope, which uses advanced image analysis techniques to obtain the deformability index of a large number of individual cells subjected to simple shear flow. Results. The RBC-DD of healthy volunteers is close to a normal distribution. In the investigated patients, distributions were markedly different and yielded significant changes in the mean, in the standard deviation, or in both. The presence of hypodeformable and hyperdeformable cell fractions can qualitatively and quantitatively be assessed from the deformability distribution (DD). In elliptocytosis, cells orient differently with respect to the streamlines, compared to normal cells. This causes the DD to be biased. Conclusions. The RBC-DD is a powerful representation to establish subpopulations with anomalous deformability. Fractions of hypodeformable and hyperdeformable cells and the standard deviation of the DD are new and excellent quantitative parameters to assess alterations in RBC deformability. (C) 2002 Elsevier Science (USA).