Temporal Stability of the Salivary Microbiota in Oral Health.
Temporal Stability of the Salivary Microbiota in Oral Health.
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DOI：
10.1371/journal.pone.0147472
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Paster BJ
中科院分区：
文献类型：
--
作者：
Belstrøm D;Holmstrup P;Bardow A;Kokaras A;Fiehn NE;Paster BJ
唾液是适合于生物标志物分析的生物流体，并且已经报道了口腔健康和疾病中唾液微生物群的差异。对于这样的比较分析，采样时间是关键的，因为细菌组成可能在一天中变化，即，日变化本研究的目的是比较唾液微生物组随时间的变化，以确定采样的最佳时间。从5名口腔健康个体以4小时间隔收集刺激唾液样品24小时，并在7天后重复收集（每人样品数量，n = 12，样品总数，n = 60）。使用使用下一代测序的人类口腔微生物鉴定（HOMINGS）分析唾液微生物群，并且使用Kruskal-Wallis检验和Benjamini-Hochberg校正进行多重比较、聚类分析、主成分分析和对应分析进行统计分析。从总共60个唾液样品中，共鉴定出477个探针靶标，每个样品的平均探针数目为207（范围：153-307）。随着时间的推移，受试者体内的微生物谱几乎没有变化或没有变化。尽管受试者之间存在相当大的差异，但受试者体内的微生物谱在24小时内和1周后保持稳定。由于唾液微生物组的昼夜变化很少或没有证据，因此唾液采样时间对于扰动或其他微生物研究并不重要。
Saliva is a biological fluid suitable for biomarker analysis, and differences in the salivary microbiota in oral health and disease have been reported. For such comparative analyses, time of sampling is critical since the bacterial composition may vary throughout the day, i.e., diurnal variation. The purpose of this study is to compare the salivary microbiome over time to determine the optimal time for sampling. Stimulated saliva samples were collected from 5 orally healthy individuals in 4 h intervals for 24 h, and collection was repeated 7 days later (number of samples per person, n = 12, total number of samples, n = 60). Salivary microbiota was analyzed using the Human Oral Microbe Identification using Next Generation Sequencing (HOMINGS), and statistical analysis was performed using the Kruskal-Wallis test with Benjamini-Hochberg’s correction for multiple comparisons, cluster analysis, principal component analysis and correspondence analysis. From a total of 60 saliva samples, 477 probe targets were collectively identified with a mean number of probes per sample of 207 (range: 153–307). Little or no variation in microbial profiles within subjects was observed over time. Although there was considerable variation between subjects, microbial profiles within subjects were stable throughout a 24 hour period and after 1 week. Since there is little or no evidence of diurnal variation of the salivary microbiome, time of sampling of saliva is not critical for perturbation or other microbial studies.