QTLs maintaining grain fertility under salt stress detected by exome QTL-seq and interval mapping in barley
QTLs maintaining grain fertility under salt stress detected by exome QTL-seq and interval mapping in barley
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DOI：
10.1270/jsbbs.18082
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发表时间：
2018-12-01
期刊：
影响因子：
2.4
通讯作者：
Hirasawa, Tadashi
中科院分区：
文献类型：
--
作者：
Kodama, Asuka;Narita, Ryouhei;Hirasawa, Tadashi
提高对盐胁迫的耐受性是增加全球粮食产量的关键战略。我们此前发现，长期盐分胁迫显著降低了盐敏感大麦品种‘OUC613’的籽粒育性，但在耐盐品种‘OUE812’中没有显著降低籽粒肥力，导致籽粒产量差异较大。在此，我们研究了这些材料在从苗期到收获期在150 mM或200 mM盐长期处理下籽粒肥力差异的潜在原因，并确定了维持籽粒肥力的数量性状基因座(QTL)。在两个材料的人工授粉试验中，只有OUC613的籽粒育性显著降低，表明OUC613籽粒育性低的主要原因是花粉育性降低。利用OUE812和OUC613杂交组合衍生的重组自交系，利用QTL-seq结合外显子捕获测序和复合区间定位，在2H染色体上定位了一个控制籽粒育性的QTL(QRP-2HB)。QTL区域包括两个编码F-box蛋白和TIFY蛋白的基因，这两个基因与雄性不育有关，突显了该区域对维持盐胁迫下的谷物育性的重要性。
Enhancing salt stress tolerance is a key strategy for increasing global food production. We previously found that long-term salinity stress significantly reduced grain fertility in the salt-sensitive barley (Hordeum vulgare) accession, 'OUC613', but not in the salt-tolerant accession, 'OUE812', resulting in large differences in grain yield. Here, we examined the underlying causes of the difference in grain fertility between these accessions under long-term treatment with 150 or 200 mM NaCl from the seedling stage to harvest and identified quantitative trait loci (QTLs) for maintaining grain fertility. In an artificial pollination experiment of the two accessions, grain fertility was significantly reduced only in OUC613 plants produced using pollen from plants grown under NaCl stress, suggesting that the low grain fertility of OUC613 was mainly due to reduced pollen fertility. Using QTL-seq combined with exome-capture sequencing and composite interval mapping of recombinant inbred lines derived from a cross between OUE812 and OUC613, we identified a QTL (qRP-2Hb) for grain fertility on chromosome 2H. The QTL region includes two genes encoding an F-box protein and a TIFY protein that are associated with male sterility, highlighting the importance of this region for maintaining grain fertility under salt stress.