Surface modification of a polyethylene film for anticoagulant and anti-microbial catheter.
Surface modification of a polyethylene film for anticoagulant and anti-microbial catheter.
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DOI：
10.1016/j.reactfunctpolym.2016.01.013
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发表时间：
2016-03-01
影响因子：
5.1
通讯作者：
Linhardt RJ
中科院分区：
文献类型：
--
作者：
Zheng Y;Miao J;Zhang F;Cai C;Koh A;Simmons TJ;Mousa SA;Linhardt RJ
介绍了一种用于聚乙烯（PE）薄膜的功能性抗凝血抗菌涂层。该纳米复合材料表面涂层的逐步制备涉及PE膜的O2等离子体蚀刻、半胱胺与蚀刻的PE膜的碳二亚胺偶联、Ag与半胱胺的巯基的结合以及肝素封端的AgNP在PE膜上的组装。采用核磁共振氢谱、衰减全反射-傅里叶变换红外光谱、X射线光电子能谱和场发射扫描电子显微镜对纳米复合膜及其组分进行了表征。所得PE膜使用血红蛋白全血凝固测定证明抗凝血活性，以及对蜡状芽孢杆菌3551（革兰氏阳性）和大肠杆菌BL 21（革兰氏阴性）细菌的抗菌活性。通过接触角测量确定肝素涂覆的PE的亲水性;并且通过原子吸收光谱法评估纳米复合材料膜相对于Ag浸出的稳定性。
A functional anticoagulant and anti-bacterial coating for polyethylene (PE) films is described. The stepwise preparation of this nanocomposite surface coating involves O2 plasma etching of PE film, carbodiimide coupling of cysteamine to the etched PE film, binding of Ag to sulfhydryl groups of cysteamine, and assembly of heparin capped AgNPs on the PE film. The nanocomposite film and its components were characterized by 1H-nuclear magnetic resonance spectroscopy, attenuated total reflectance-Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and field emission-scanning electron microscopy. The resulting PE films demonstrate anticoagulant activity using a hemoglobin whole blood clotting assay, and anti-bacterial activity against Bacillus cereus 3551 (Gram-positive) and Escherichia coli BL21 (Gram-negative) bacteria. The hydrophilicity of the heparin coated PE was determined by contact angle measurements; and the stability of the nanocomposite film, with respect to Ag leaching, was assessed by atomic absorption spectroscopy.