Facile and sensitive detection of influenza viruses using SERS antibody probes
Facile and sensitive detection of influenza viruses using SERS antibody probes
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DOI：
10.1039/c6ra13966c
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发表时间：
2016-01-01
期刊：
影响因子：
3.9
通讯作者：
Kang, Taejoon
中科院分区：
文献类型：
--
作者：
Moon, Jeong;Yi, So Yeon;Kang, Taejoon
快速、准确地识别流感病毒对于预防未来的大流行疫情非常重要。建立了一种简单、灵敏的流感病毒表面增强拉曼散射（SERS）抗体探针检测方法。通过将金纳米粒子、金结合肽-蛋白G和抗体混合制备SERS抗体探针，无需复杂的化学或生物反应。它们还保留了抗体与流感病毒A/CA/07/2009 （pH1N1）相互作用的最佳构象，使我们能够敏感和选择性地检测pH1N1。该方法检出限为4.1 × 10(3) TCID / mL，对pH1N1具有高选择性。我们预计，这种方法可以在广泛的免疫分析有用。
It is highly important to identify influenza viruses rapidly and accurately for the prevention of future pandemic outbreaks. We developed an easy and sensitive influenza virus detection method using surface-enhanced Raman scattering (SERS) antibody probes. The SERS antibody probes can be prepared easily by mixing Au nanoparticles, gold binding peptide-protein G, and antibodies without complicated chemical or biological reactions. They also retain the optimal conformation for the antibody's interaction with Influenza A/CA/07/2009 (pH1N1), allowing us to detect pH1N1 sensitively and selectively. This method provides a detection limit of 4.1 x 10(3) TCID per mL and is highly selective for pH1N1. We anticipate that this method can be useful in a wide range of immunoassays.