A single base pair dominates over the novel identity of an Escherichia coli tyrosine tRNA in Saccharomyces cerevisiae.
A single base pair dominates over the novel identity of an Escherichia coli tyrosine tRNA in Saccharomyces cerevisiae.
复制标题
酿酒酵母中大肠杆菌酪氨酸 tRNA 的新特性由单碱基对主导。
DOI：
10.1128/mcb.11.5.2744-2751.1991
复制
发表时间：
1991
影响因子：
5.3
通讯作者：
Schimmel,P
中科院分区：
文献类型：
--
作者：
Trézéguet,V;Edwards,H;Schimmel,P
大肠杆菌su+3酪氨酸tRNA在酿酒酵母中是一种亮氨酸特异性tRNA，这一发现提出了大肠杆菌中tRNA同一性的某些决定因素可能是由于大肠杆菌su +3酪氨酸tRNA在酿酒酵母中的存在而引起的。为了研究酵母菌系统是否对大肠杆菌中丙氨酸接受的主要决定因素敏感，在大肠杆菌中，将一个G3 · U70碱基对引入到该u +3酪氨酸tRNA的受体螺旋中。这个替换转换了E的身份。大肠杆菌抑制子从亮氨酸变为丙氨酸。，如。在大肠杆菌中，G3 U70是丙氨酸接受的强决定因子，其可以支配可能被替代性充电酶识别的tRNA中的其他特征。
TheEscherichia coli su+3tyrosine tRNA was shown recently to be a leucine-specific tRNA inSaccharomyces cerevisiae.This finding raises the possibility that some determinants for tRNA identity inE. colimay be different inS. cerevisiae.To investigate whether the fungal system is sensitive to the major determinant for alanine acceptance inE. coli,a single G3 • U70 base pair was introduced into the acceptor helix of thesu+3tyrosine tRNA. This substitution converts the identity of theE. colisuppressor inS. cerevisiaefrom leucine to alanine. Thus, as inE. coli,G3 U70 is a strong determinant for alanine acceptance that can dominate over other features in a tRNA that might be recognized by alternative charging enzymes.