Distinct expression patterns of the subunits of the CCR4-NOT deadenylase complex during neural development
Distinct expression patterns of the subunits of the CCR4-NOT deadenylase complex during neural development
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神经发育过程中 CCR4-NOT 去腺苷酸酶复合物亚基的独特表达模式
DOI：
10.1016/j.bbrc.2011.06.148
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发表时间：
2011-07-29
影响因子：
3.1
通讯作者：
Yokoyama, Kazumasa
中科院分区：
文献类型：
--
作者：
Chen, Chuan;Ito, Kentaro;Yokoyama, Kazumasa
基因表达的稳定性影响基因表达的动态化。哺乳动物的CCR4-NOT复合体与死烯基酶活性有关，该酶活性缩短了mRNA聚(A)尾，从而导致mRNAs的不稳定。该复合体至少由9个亚基组成，在HeLa细胞中主要形成一个2.0的丙二醛蛋白复合体。越来越多的证据表明，CCR4-NOT复合体参与了细胞的生长和存活，但其生物活性的调控机制尚不清楚。在这里，我们分析了CCR4-NOT复合体的亚单位在小鼠不同组织中的表达水平，发现它们表现出不同的表达模式。CNOT6、6L、7和10几乎无处不在地表达，而其他的以组织特异性的方式表达，例如那些在大脑中表现出特别高表达的表达。此外，CNOT2、3、6和8在神经干细胞分化过程中迅速下调。这些发现表明，CCR4-NOT复合体的亚基组成在不同组织中不同，并在神经发育过程中发生变化，从而在基因表达控制方面赋予了额外的一层特异性。(C)2011 Elsevier Inc.保留所有权利。
The stability of mRNA influences the dynamics of gene expression. The mammalian CCR4-NOT complex is associated with deadenylase activity, which shortens the mRNA poly(A) tail and thereby contributes to destabilization of mRNAs. The complex consists of at least nine subunits and predominantly forms a 2.0 MDa protein complex in HeLa cells. Accumulating evidence suggests that the CCR4-NOT complex is involved in cell growth and survival; however, the regulatory mechanisms of its biological activity remain obscure. Here, we analyzed the expression levels of the subunits of the CCR4-NOT complex in various mouse tissues and found that they showed distinct expression patterns. CNOT6, 6L, 7, and 10 were expressed nearly ubiquitously, whereas others were expressed in tissue-specific manners, such as those displaying especially high expression in the brain. Furthermore, CNOT2, 3, 6, and 8 were rapidly downregulated during differentiation of neural stem cells. These findings suggest that subunit composition of the CCR4-NOT complex differs among tissues and is altered during neural development, thereby imparting an additional layer of specificity in the control of gene expression. (C) 2011 Elsevier Inc. All rights reserved.